tRNA wobble modification affects leaf cell development in Arabidopsis thaliana.
tRNA wobble modification affects leaf cell development in Arabidopsis thaliana.
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DOI：
10.1093/pcp/pcz064
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发表时间：
2019-09
影响因子：
4.9
通讯作者：
Y. Nakai;G. Horiguchi;Kosei Iwabuchi;A. Harada;M. Nakai;I. Hara-Nishimura;T. Yano
中科院分区：
文献类型：
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作者：
Y. Nakai;G. Horiguchi;Kosei Iwabuchi;A. Harada;M. Nakai;I. Hara-Nishimura;T. Yano
在Lys、Glu和Gln的摆动位置处的tRNA修饰（摆动U * 修饰）负责蛋白质翻译效率和翻译速率的微调。这种修饰根据生长和环境变化影响生物体功能。然而，WobbleU* 修饰在细胞、组织或个体水平上的影响尚未阐明。在这项研究中，我们表明硫修饰的wobbleU* 的tRNA影响拟南芥叶片发育。硫修饰受损的两个wobbleU*-修饰突变体：URM 1样蛋白缺陷型突变体和延伸复合物缺陷型突变体。对突变体表型的分析表明，wobbleU* 修饰的缺陷增加了叶片中的气隙和叶片大小，而不影响栅栏叶肉细胞的数量和面积。另一方面，这两个突变体表现出叶表皮铺装细胞的数量增加，但细胞大小减少。wobbleU* 修饰的缺陷也延迟了叶肉细胞核内复制过程的启动。不对称叶2缺陷突变体的表型在延伸子缺陷突变体中增强，而在URM 1样蛋白缺陷突变体中没有变化。总的来说，这项研究的结果表明，tRNA wobbleU* 修改起着重要的作用，在叶形态建成之间的平衡表皮和叶肉组织的发展。
The tRNA modification at the wobble position of Lys, Glu, and Gln (wobbleU* modification is responsible for the fine-tuning of protein translation efficiency and translation rate. This modification influences organism function in accordance with growth and environmental changes. However, the effects of wobbleU* modification at the cellular, tissue, or individual level have not yet been elucidated. In this study, we show that sulfur modification of wobbleU* of the tRNAs affects leaf development in Arabidopsis thaliana. The sulfur modification was impaired in the two wobbleU*-modification mutants: the URM1-like protein-defective mutant and the Elongator complex-defective mutants. Analyses of the mutant phenotypes revealed that the deficiency in the wobbleU* modification increased the airspaces in the leaves and the leaf size without affecting the number and the area of palisade mesophyll cells. On the other hand, both mutants exhibited increased number of leaf epidermal pavement cells but with reduced cell size. The deficiency in the wobbleU* modification also delayed the initiation of the endoreduplication processes of mesophyll cells. The phenotype of ASYMMETRIC LEAVES2-defective mutant was enhanced in the Elongator-defective mutants, while it was unchanged in the URM1-like protein-defective mutant. Collectively, the findings of this study suggest that the tRNA wobbleU* modification plays an important role in leaf morphogenesis by balancing the development between epidermal and mesophyll tissues.