Rat Monoclonal Antibody Specific for MyoD
Rat Monoclonal Antibody Specific for MyoD
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DOI：
10.1089/hyb.2009.0117
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发表时间：
2010-06-01
期刊：
影响因子：
--
通讯作者：
Tachibana, Taro
中科院分区：
文献类型：
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作者：
Harada, Akihito;Ohkawa, Yasuyuki;Tachibana, Taro
肌生成决定因子1（MyoD）是一种肌生成调节因子（MRF），具有碱性结构域和螺旋-环-螺旋结构域。MRF在成肌细胞命运和终末分化中起关键作用。MyoD是一种转录因子，通过与骨骼肌中表达的基因调控因子结合来诱导转录。作为主基因，MyoD还决定骨骼肌分化。在这项研究中，我们建立了一个单克隆抗体特异性MyoD使用大鼠髂内淋巴结的方法。免疫印迹分析表明，我们的单克隆抗体对MyoD可以识别全长MyoD。此外，免疫细胞化学染色显示在骨骼肌分化阶段MyoD的表达变化。这种针对MyoD的单克隆抗体允许进一步研究以阐明MyoD影响骨骼肌分化的机制。
Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.