Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.
Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.
复制标题
影响酿酒酵母 tRNA 剪接核酸内切酶活性的突变。
作者：
Mark Winey;M. Culbertson
已经在酵母中鉴定了两种改变tRNA剪接核酸内切酶的酶活性的非连锁突变。定位于12号染色体上的sen 1 -1突变导致温度敏感性生长、体外核酸内切酶活性降低和未剪接的前体tRNA的体内积累。sen 2 -1突变不会导致可检测的生长缺陷，但会导致体外核酸内切酶活性的温度依赖性降低。前体tRNA在sen 2 -1菌株中不积累。在来自杂合二倍体的提取物中，sen 1 -1和sen 2 -1的体外酶活性互补，但在来自单独的sen 1 -1和sen 2 -1单倍体菌株的混合提取物中不能互补。这些结果表明SEN基因产物在酶促去除tRNA内含子中的直接作用，这与已知影响tRNA剪接的其他产物的作用不同。
Two unlinked mutations that alter the enzyme activity of tRNA-splicing endonuclease have been identified in yeast. The sen1-1 mutation, which maps on chromosome 12, causes temperature-sensitive growth, reduced in vitro endonuclease activity, and in vivo accumulation of unspliced pre-tRNAs. The sen2-1 mutation does not confer a detectable growth defect, but causes a temperature-dependent reduction of in vitro endonuclease activity. Pre-tRNAs do not accumulate in sen2-1 strains. The in vitro enzyme activities of sen1-1 and sen2-1 complement in extracts from a heterozygous diploid, but fail to complement in mixed extracts from separate sen1-1 and sen2-1 haploid strains. These results suggest a direct role for SEN gene products in the enzymatic removal of introns from tRNA that is distinct from the role of other products known to affect tRNA splicing.
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DOI：
10.1073/pnas.81.9.2616
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DINARDO, S;VOELKEL, K;STERNGLANZ, R
通讯作者：
STERNGLANZ, R
鉴定缺乏 DNA 拓扑异构酶 I 活性的酿酒酵母突变体。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thrash,C;Voelkel,K;DiNardo,S;Sternglanz,R
通讯作者：
Sternglanz,R
酵母脯氨酸 tRNA 的剪接，其中反密码子茎中含有新的抑制突变。
影响因子：
5.6
作者：
Winey,M;Mendenhall,MD;Cummins,CM;Culbertson,MR;Knapp,G
通讯作者：
Knapp,G
影响因子：
3.3
作者：
Gaber,RF;Culbertson,MR
通讯作者：
Culbertson,MR