Assays for autophagy II: Mitochondrial autophagy.
Assays for autophagy II: Mitochondrial autophagy.
复制标题
自噬测定 II：线粒体自噬。
DOI：
10.1007/978-1-4939-0799-1_11
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Okamoto K.
中科院分区：
文献类型：
--
作者：
Kanki T;Okamoto K.
自噬是细胞质蛋白和细胞器通过溶酶体/空泡机制进行的大量降解过程。线粒体自噬是一种选择性降解线粒体的自噬。近年来的研究表明，线粒体自噬在细胞线粒体质量控制中起着重要作用。酿酒酵母是一种强大的模型，已被应用于许多生物现象的研究。这种模式生物对我们对自噬的理解做出了巨大贡献，包括鉴定了30多个自噬相关基因。同样，线粒体自噬的分子机制和生理作用也逐渐被阐明。酵母。在本章中，我们描述了检测有丝分裂inS的两种常用协议。酿酒酵母：荧光显微镜和免疫印迹。
Autophagy is a bulk degradation process of cytosolic proteins and organelles through the lysosomal/vacuolar machinery. Mitophagy is a type of autophagy that selectively degrades mitochondria. Recent studies have revealed that mitophagy plays an important role in cellular mitochondrial quality control. The budding yeastSaccharomyces cerevisiaeis a powerful model that has been applied to study many biological phenomena. This model organism has contributed greatly to our understanding of autophagy, including the identification of more than 30 autophagy-related genes. Similarly, the molecular mechanisms and physiological roles of mitophagy have been gradually elucidated usingS. cerevisiae. In this chapter, we describe two commonly used protocols to detect mitophagy inS. cerevisiae: fluorescence microscopy and immunoblotting.