Cationic amphiphile in phospholipid bilayer or oil-water interface of nanocarriers affects planktonic and biofilm bacteria killing
Cationic amphiphile in phospholipid bilayer or oil-water interface of nanocarriers affects planktonic and biofilm bacteria killing
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DOI：
10.1016/j.nano.2016.08.011
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发表时间：
2017-02-01
影响因子：
5.4
通讯作者：
Fang, Jia-You
中科院分区：
文献类型：
--
作者：
Lin, Ming-Hsien;Hung, Chi-Feng;Fang, Jia-You
制备了一种阳离子两亲物大豆乙基吗啉乙基硫酸盐（SME），并将其固定在脂质体或纳米乳液中，比较了SME嵌入磷脂双层膜和油水界面的抗菌活性。在抗菌评估之前，脂质体和纳米乳剂的尺寸分别记录为75和214 nm。最小抑菌浓度（MIC）/最小杀菌浓度（MBC）和活/死细胞计数的数据表明，与脂质体相比，纳米乳对金黄色葡萄球菌、耐甲氧西林链球菌具有上级的抗菌活性。金黄色葡萄球菌（MRSA）和表皮葡萄球菌。纳米乳液孵育使生物膜厚度降低2.4倍，而脂质体显示厚度降低1.6倍。发现SME插入油-水相中诱导细菌膜破坏。SME纳米系统对角质形成细胞无毒。阳离子纳米系统的体内局部应用减少了小鼠的皮肤感染、MRSA负荷和炎症。由MRSA引起的恶化的皮肤屏障功能通过纳米乳治疗得以恢复。(C)2016 Elsevier Inc. All rights reserved.
A cationic amphiphile, soyaethyl morpholinium ethosulfate (SME), immobilized in liposomes or nanoemulsions, was prepared in an attempt to compare the antibacterial activity between SME intercalated in the phospholipid bilayer and oil-water interface. Before antibacterial assessment, the size of the liposomes and nanoemulsions was respectively recorded as 75 and 214 nm. The data of minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC) and live/dead cell count demonstrated a superior antimicrobial activity of nanoemulsions compared to liposomes against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and Staphylococcus epidermidis. Nanoemulsion incubation reduced biofilm thickness by 2.4-fold, whereas liposomes showed a 1.6-fold decrease in thickness. SME insertion in the oil-water phase was found to induce bacterial membrane disruption. SME nanosystems were nontoxic to keratinocytes. In vivo topical application of the cationic nanosystems reduced skin infection, MRSA load, and inflammation in mice. The deteriorated skin barrier function evoked by MRSA was recovered by nanoemulsion treatment. (C) 2016 Elsevier Inc. All rights reserved.