Presence of Lactobacillus reuteri in saliva coincide with higher salivary IgA in young adults after intake of probiotic lozenges
Presence of Lactobacillus reuteri in saliva coincide with higher salivary IgA in young adults after intake of probiotic lozenges
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DOI：
10.3920/bm2016.0081
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发表时间：
2017-01-01
影响因子：
5.4
通讯作者：
Jorgensen, M. R.
中科院分区：
文献类型：
--
作者：
Braathen, G.;Ingildsen, V.;Jorgensen, M. R.
本研究的目的是比较在使用益生菌含片干预三周后，唾液中存在和不存在罗伊氏乳杆菌的年轻人唾液免疫球蛋白A （IgA）和选定的白细胞介素(IL)-1 β、IL-6、IL-8和IL-10的浓度。研究小组由47名年龄在18-32岁的健康个体组成，没有口腔炎症的临床症状。在一项随机、双盲、安慰剂对照的交叉试验中，参与者每天服用两种含两种益生菌罗伊氏乳杆菌的含片或安慰剂含片。干预期和洗脱期为三周。在基线和干预期结束后立即收集受刺激和未受刺激的全唾液。分析样品的总蛋白、唾液IgA和选定的细胞因子。在这项扩展分析中，通过dna提取、pcr扩增和凝胶电泳分析基线和随访唾液样本中与摄入益生菌含片有关的罗伊氏乳杆菌的存在，收集数据。在基线时，27%的人显示出罗伊氏乳杆菌的存在，42%的人在三周的益生菌干预后立即呈阳性。唾液中存在罗伊氏乳杆菌的个体，其P
The aim of this study was to compare the concentration of salivary immunoglobulin A (IgA) and the selected interleukins (IL)-1 beta, IL-6, IL-8 and IL-10 in young individuals with presence and non-presence of Lactobacillus reuteri in saliva after a three-week intervention with probiotic lozenges. The study group consisted of 47 healthy individuals aged 18-32 years with no clinical signs of oral inflammation. In a randomised, double-blind, placebo-controlled, cross-over trial participants ingested two lozenges per day containing two strains of the probiotic bacterium L. reuteri or placebo lozenges. The intervention and wash-out periods were three weeks. Stimulated and unstimulated whole saliva was collected at baseline and immediately after termination of the intervention periods. The samples were analysed for total protein, salivary IgA and selected cytokines. In this extended analysis, data were collected by analysing baseline and follow-up saliva samples related to ingestion of the probiotic lozenges for the presence of L. reuteri through DNA-extraction, PCR-amplification and gel-electrophoresis. At baseline, 27% of the individuals displayed presence of L. reuteri and 42% were positive immediately after the three-week probiotic intervention. Individuals with presence of L. reuteri in saliva had significantly higher (P