Developmentally regulated expression of cyclin D3 and its potential in vivo interacting proteins during murine gametogenesis.
Developmentally regulated expression of cyclin D3 and its potential in vivo interacting proteins during murine gametogenesis.
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DOI：
10.1210/endo.140.6.6756
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发表时间：
1999-06
期刊：
影响因子：
4.8
通讯作者：
Qi Zhang;Xiangyuan Wang;D. Wolgemuth
中科院分区：
文献类型：
--
作者：
Qi Zhang;Xiangyuan Wang;D. Wolgemuth
为了开始评估细胞周期调节因子cyclin D3在配子发生过程中的功能，本研究检测了其在小鼠睾丸和卵巢中的表达、相互作用伙伴和相关激酶活性。在生后睾丸发育的早期阶段，在精原细胞和间质细胞中检测到cyclinD3蛋白。在成年睾丸中，cyclin D3也在终末分化的精子细胞中表达。在胚胎卵巢中，细胞周期蛋白D3的检测仅限于体细胞。在出生后的卵巢中，其定位主要是在原始卵泡和小卵泡的卵母细胞的核中，随着卵母细胞的生长而减少。Cdk4和p27在睾丸和卵巢细胞的相似亚群中表达，这表明它们可能以细胞类型特异性的方式在睾丸和卵巢发育过程中调节细胞周期蛋白D3的功能。在未成熟的睾丸和卵巢中检测到细胞周期蛋白D3相关激酶活性，但在成年睾丸和卵巢中未检测到。这些观察结果表明，细胞周期蛋白D3在控制细胞分裂和分化的生殖细胞系和有丝分裂和减数分裂细胞周期的差异调节男性和女性配子发生的独特作用。
To begin to assess the function of the cell cycle regulator cyclin D3 during gametogenesis, the present study examined its expression, interacting partners, and associated kinase activity in the murine testis and ovary. In the early stages of postnatal testicular development, cyclin D3 protein was detected in spermatogonia and Leydig cells. In the adult testis, cyclin D3 was also expressed in terminally differentiating spermatids. In the embryonic ovary, detection of cyclin D3 was limited to somatic cells. In the postnatal ovary, its localization was predominantly in the nuclei of oocytes in primordial and small follicles, a localization that diminished with oocyte growth. Cdk4 and p27 were expressed in a similar subset of testicular and ovarian cells, suggesting that they may regulate cyclin D3 function during testicular and ovarian development in a cell type-specific manner. Cyclin D3-associated kinase activity was detected in immature, but not adult, testes and ovaries. These observations suggest unique roles for cyclin D3 in the control of cell division and differentiation in the germ line and the differential regulation of mitotic and meiotic cell cycles during male and female gametogenesis.