Sperm-mediated Repoter Gene gfp and egfp Transfer in the Silkworm
Sperm-mediated Repoter Gene gfp and egfp Transfer in the Silkworm
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发表时间：
2006
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通讯作者：
Hong Man-xian
中科院分区：
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作者：
Hong Man-xian
In this study,the effiency of sperm-mediated gene transfer was analyzed for foreign gene introduced into and intergrated in the silkworm′s genome and inherited by the offspring.Different concentration or conformation vector DNA including gfp or egfp report gene were used in the gene transfer experiment on silkworm.The results showed that 5 DNA samples extracted from 14 G_(0)generation spawns were positive by PCR(positive proportion 35.7%) after introduction linearized pG350 with the concentration 2.0 mg/mL into moth,and the results of PCR and Southern blot detection of G_1 generation indicated that the gfp gene was intergrated in the silkworm′s genome.The positive proportion of introduction linearized or circular pMD-Fib-IE-EGFP was 61.2% and 57.1% by PCR respectively.When the circular pEGFP-N3 was transferred with the concentration 1.2 mg/mL,the positive proportion was(46.2)% by PCR.The hybridization results indicated that the egfp gene had intergrated in the genome of G_0 generation silkworm.From this study,the conclusion was drawn that the sperm-mediated gene transfer technology could introduce foreign gene into silkworm,and the foreign gene also could intergrated in the genome and transmitted to G_1 generation.
