Overexpression of the glucose transporter gene with a Herpes simplex viral vector protects striatal neurons against stroke
Overexpression of the glucose transporter gene with a Herpes simplex viral vector protects striatal neurons against stroke
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DOI：
10.1097/00004647-199603000-00001
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发表时间：
1996-03-01
影响因子：
6.3
通讯作者：
Steinberg, GK
中科院分区：
文献类型：
--
作者：
Lawrence, MS;Sun, GH;Steinberg, GK
携带葡萄糖转运蛋白（GT）基因和标记基因的单纯疱疹病毒载体被发现可以保护神经元免受1小时局灶性缺血损伤。接受GT载体v α 22 β gal α 4GT的大鼠与对侧对照组（n = 7）相比，在缺血半球中表现出67.4 +/-35.3%的病毒靶向神经元存活率，而接受对照载体的大鼠仅表现出32.8 +/-17.9%的存活率（n = 9）。存活率的显著改善（105%，p = 0.022）表明能量衰竭是纹状体缺血性损伤神经病理学的重要因素，并且可以通过基因转移缓解。这是第一次证明通过将GT基因直接转移到神经元来保护缺血性脑损伤。
Herpes simplex virus vectors bearing a glucose transporter (GT) gene and a marker gene were found to protect neurons against a 1-h focal ischemic insult. Rats receiving the GT vector v alpha 22 beta gal alpha 4GT exhibited a 67.4 +/- 35.3% survival of virally targeted neurons in the ischemic hemisphere compared with the contralateral control (n = 7), whereas rats receiving a control vector exhibited only 32.8 +/- 17.9% survival (n = 9). This significant improvement in survival(105%, p = 0.022) suggests that energy failure is an important contributor to the neuropathology of ischemic damage in the striatum, and that it can be alleviated by gene transfer. This is the first demonstration of protection against ischemic cerebral injury by the direct transfer of GT genes to neurons.