The proteasome controls ESCRT-III-mediated cell division in an archaeon
The proteasome controls ESCRT-III-mediated cell division in an archaeon
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DOI：
10.1126/science.aaz2532
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发表时间：
2020-08-07
期刊：
影响因子：
56.9
通讯作者：
Baum, Buzz
中科院分区：
文献类型：
--
作者：
Risa, Gabriel Tarrason;Hurtig, Fredrik;Baum, Buzz
Sulfolobus acidocalarius是真核生物实验中最接近的古细菌亲戚，尽管缺乏明显的周期蛋白依赖性激酶和周期蛋白同源物，但具有有序的真核细胞样细胞周期，具有不同的DNA复制和分裂阶段。在本文中，为了探索s.acidocalarius的细胞分裂机制，我们确定了古细菌蛋白酶体在调节从一个细胞周期结束到下一个细胞周期开始的转变中的作用。此外，我们确定了古细菌ESCRT-III同源物CdvB作为蛋白酶体的关键靶点，并表明其降解通过允许收缩CdvB1:CdvB2 ESCRT-III分裂环来触发分裂。这些发现提供了escrt - iii介导的膜重塑的最小机制，并指出蛋白酶体在真核和古细菌细胞周期控制中的保守作用。
Sulfolobus acidocaldarius is the closest experimentally tractable archaeal relative of eukaryotes and, despite lacking obvious cyclin-dependent kinase and cyclin homologs, has an ordered eukaryote-like cell cycle with distinct phases of DNA replication and division. Here, in exploring the mechanism of cell division in S. acidocaldarius, we identify a role for the archaeal proteasome in regulating the transition from the end of one cell cycle to the beginning of the next. Further, we identify the archaeal ESCRT-III homolog, CdvB, as a key target of the proteasome and show that its degradation triggers division by allowing constriction of the CdvB1:CdvB2 ESCRT-III division ring. These findings offer a minimal mechanism for ESCRT-III-mediated membrane remodeling and point to a conserved role for the proteasome in eukaryotic and archaeal cell cycle control.