RNF151, a testis-specific RING finger protein, interacts with dysbindin.
RNF151, a testis-specific RING finger protein, interacts with dysbindin.
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DOI：
10.1016/j.abb.2007.05.013
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发表时间：
2007-09
影响因子：
3.9
通讯作者：
Hongqiang Nian;Cuihong Fan;Shangying Liao;Yuqiang Shi;Keying Zhang;Yi-Xun Liu;Chunsheng Han
中科院分区：
文献类型：
--
作者：
Hongqiang Nian;Cuihong Fan;Shangying Liao;Yuqiang Shi;Keying Zhang;Yi-Xun Liu;Chunsheng Han
环指蛋白在精子发生过程中起重要作用。在这里，我们报告了一种新的RING指蛋白RNF 151，具有C3 HC 4型RING指结构域，推定的核定位信号（NLS），和TRAF型锌指结构域，专门在小鼠睾丸中表达，并在精子发生过程中发育调节。虽然RNF 151 mRNA存在于圆形精子细胞中，但其蛋白质在第VIII-IX期曲细精管的伸长精子细胞中表达。NLS和RING结构域是RNF 151-EGFP在转染细胞中的核定位所必需和充分的。酵母双杂交筛选鉴定了小鼠RNF 151和dysbindin的物理相互作用，这通过蛋白质的共免疫沉淀和它们在完整细胞中的共定位来证实。由于dysbindin最近被证明参与膜生物发生和融合，顶体形成的关键过程，我们建议RNF 151可能在顶体形成中发挥作用。
RING finger proteins play important roles in spermatogenesis. Here, we report that a novel RING finger protein RNF151, with a C3HC4-type RING finger domain, a putative nuclear localization signal (NLS), and a TRAF-type zinc finger domain, was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII–IX seminiferous tubules. The NLS together with the RING domain were necessary and sufficient for the nuclear localization of RNF151-EGFP in transfected cells. Yeast two-hybrid screening identified the physical interaction of mouse RNF151 and dysbindin, which was confirmed by the co-immunoprecipitation of the proteins and by their co-localization in intact cells. As dysbindin has lately been shown to be involved in membrane biogenesis and fusion, a key process for acrosome formation, we propose that RNF151 may play a role in acrosome formation.