Effects of HMGB1 on ischemia-reperfusion injury in the rat heart
Effects of HMGB1 on ischemia-reperfusion injury in the rat heart
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DOI：
10.1253/circj.72.1178
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Sano, Shunji
中科院分区：
文献类型：
--
作者：
Oozawa, Susumu;Mori, Shuji;Sano, Shunji
背景冠状动脉缺血再灌注（I/R）损伤通过包括炎症反应在内的多步骤过程导致心肌细胞坏死。最近的一项研究表明，高迁移率族蛋白 1 (HMGB 1) 是致死性败血症的晚期介质，也是 I/R 损伤后炎症和坏死的早期介质。在本研究中，使用针对 HMGB1 的中和单克隆抗体 (mAb) 来阐明 HMGB 1 在心脏 I/R 损伤中的作用。 方法和结果 大鼠接受 30 分钟的左冠状动脉闭塞，然后进行 60 分钟的再灌注。在再灌注前静脉注射抗 HMGB1 mAb 或对照 IgG。与对照组相比，抗 HMGB1 mAb 组的梗塞面积扩大（p
Background Coronary ischemia-reperfusion (I/R) injury causes cardiomyocyte necrosis in a multi-step process that includes an inflammatory reaction. A recent study has suggested that high-mobility group box 1 (HMGB 1) is a late mediator of lethal sepsis and an early mediator of inflammation and necrosis following I/R injury. In the present study a neutralizing monoclonal antibody (mAb) for HMGB1 was used to clarify the role of HMGB 1 in cardiac I/R injury.Methods and Results Rats underwent 30 min of left coronary artery occlusion followed by 60 min reperfusion. An intravenous injection of anti-HMGB1 mAb or control IgG was administered just before reperfusion. The infarct size was enlarged in the anti-HMGB1 mAb group in comparison with the control group (p