CD9 correlates with cancer stem cell potentials in human B-acute lymphoblastic leukemia cells
CD9 correlates with cancer stem cell potentials in human B-acute lymphoblastic leukemia cells
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DOI：
10.1016/j.bbrc.2009.02.123
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发表时间：
2009-04-24
影响因子：
3.1
通讯作者：
Morimoto, Chikao
中科院分区：
文献类型：
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作者：
Nishida, Hiroko;Yamazaki, Hiroto;Morimoto, Chikao
癌症干细胞（CSC）理论表明，只有一小部分具有干细胞样潜能的细胞亚群才能启动肿瘤的发展。虽然最近关于急性淋巴细胞白血病（ALL）的数据相互矛盾，但一些研究已经证明了在原始样本的CD 34靶向分离后存在此类细胞。虽然CD 34是分离白血病中CSC的有用标志物，但除了CD 34之外的其他特异性标志物的鉴定相对不成功。为了鉴定新的标志物，我们首先对几种B-ALL细胞系的表面标志物进行了广泛的分析。我们的数据表明，每一个B-ALL细胞系测试不表达CD 34，但某些线包含的细胞群体具有显着的异质性标志物的表达。此外，CD 9（+）细胞群具有干细胞特性的克隆，在体外和移植实验证明。这些结果表明，CD 9是一个有用的阳性选择标记物，用于识别CSC在B-ALL。(C)2009 Elsevier Inc. All rights reserved.
Cancer stem cell (CSC) theory suggests that only a small subpopulation of cells having stem cell-like potentials can initiate tumor development. While recent data on acute lymphoblastic leukemia (ALL) are conflicting, some studies have demonstrated the existence of such cells following CD34-targeted isolation of primary samples. Although CD34 is a useful marker for the isolation of CSCs in leukemias, the identification of other specific markers besides CD34 has been relatively unsuccessful. To identify new markers, we first performed extensive analysis of surface markers on several B-ALL cell lines. Our data demonstrated that every B-ALL cell line tested did not express CD34 but certain lines contained cell populations with marked heterogeneity in marker expression. Moreover, the CD9(+) cell population possessed stern cell characteristics within the clone, as demonstrated by in vitro and transplantation experiments. These results suggest that CD9 is a useful positive-selection marker for the identification of CSCs in B-ALL. (C) 2009 Elsevier Inc. All rights reserved.