Arenimonas subflava sp. nov., isolated from a drinking water network, and emended description of the genus Arenimonas.
Arenimonas subflava sp. nov., isolated from a drinking water network, and emended description of the genus Arenimonas.
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DOI：
10.1099/ijs.0.000201
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发表时间：
2015-06
影响因子：
2.8
通讯作者：
J. Makk;Z. Homonnay;Zsuzsa Kéki;Katalin Nemes-Barnás;K. Márialigeti;P. Schumann;E. Tóth
中科院分区：
文献类型：
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作者：
J. Makk;Z. Homonnay;Zsuzsa Kéki;Katalin Nemes-Barnás;K. Márialigeti;P. Schumann;E. Tóth
从匈牙利布达佩斯的饮用水管网中分离到一株命名为PYM 3 - 14 T的菌株，并通过多相分类学方法对其进行了研究。革兰氏阴性染色，直杆状，需氧，不运动。菌株PYM 3 - 14 T的16 S rRNA基因序列的系统发育分析揭示了与γ-变形菌纲内的黄单胞菌科成员的明确联系。菌株PYM 3 - 14 T的16 S rRNA基因序列与大昌砂单胞菌CH 15 - 1 T（96.2%）、米泰尔砂单胞菌YC 6267 T（95.2%）和棕色溶杆菌UASM DT（94.4%）的同源性最高。PYM 3 - 14 T的DNA G+C含量分别为52.0mol%和55.9mol%，远低于Arenimonas属的任何成员。主要脂肪酸（> 8%）为异-C16：0、异-C15：0、异-C14：0、异-C17：1ω 9 c和C16：1ω 7 c醇。菌株PYM 3 - 14 T含有Q-8作为主要的泛醌和磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和磷脂酰单甲基乙醇胺作为主要的极性脂质。根据表型和基因型数据，菌株PYM 3 - 14 T代表Arenimonas属的一个新种，建议将其命名为Arenimonasubflava sp. nov.。模式菌株为PYM 3 - 14 T（= NCAIM B 02508 T = DSM 25526 T）。根据本研究中获得的新资料，还提出了一个修正的Arenimonas属的描述。
A strain designated PYM3-14T was isolated from the drinking water network of Budapest (Hungary) and was studied by polyphasic taxonomic methods. The straight-rod-shaped cells stained Gram-negative, were aerobic and non-motile. Phylogenetic analysis of the 16S rRNA gene sequence of strain PYM3-14T revealed a clear affiliation with members of the family Xanthomonadaceae within the class Gammaproteobacteria. The 16S rRNA gene sequence of strain PYM3-14T showed the closest sequence similarities to Arenimonas daechungensis CH15-1T (96.2 %), Arenimonas oryziterrae YC6267T (95.2 %) and Lysobacter brunescens UASM DT (94.4 %). The DNA G+C content of strain PYM3-14T, measured by two different methods (52.0 mol% and 55.9 mol%, respectively), was much lower than that of any member of the genus Arenimonas. The predominant fatty acids (>8 %) were iso-C16:0, iso-C15:0, iso-C14:0, iso-C17:1ω9c and C16:1ω7c alcohol. Strain PYM3-14T contained Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. According to phenotypic and genotypic data strain PYM3-14T represents a novel species of the genus Arenimonas, for which the name Arenimonas subflava sp. nov. is proposed. The type strain is PYM3-14T ( = NCAIM B 02508T = DSM 25526T). On the basis of new data obtained in this study, an emended description of the genus Arenimonas is also proposed.