Activation of NADPH-oxidase by arachidonic acid involves phospholipase A2 in intact human neutrophils but not in the cell-free system.
Activation of NADPH-oxidase by arachidonic acid involves phospholipase A2 in intact human neutrophils but not in the cell-free system.
复制标题
花生四烯酸对 NADPH 氧化酶的激活涉及完整人中性粒细胞中的磷脂酶 A2，但在无细胞系统中则不然。
DOI：
10.1016/s0006-291x(86)80395-3
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Tauber,AI
中科院分区：
文献类型：
--
作者：
Maridonneau-Parini,I;Tauber,AI
本文研究了花生四烯酸（AA）刺激完整人中性粒细胞中NADPH氧化酶的机制，并与无细胞系统中的机制进行了比较。[3 H]-AA在AA刺激下从预先标记的细胞中释放，并且磷脂酶A2抑制剂平行地减少[3 H]-AA和超氧化物的释放。环氧合酶，脂氧合酶或蛋白激酶抑制剂未能影响这两种反应。在无细胞系统中，没有释放[3 H]-AA后观察到AA添加，而NADPH-氧化酶被激活;超氧化物的产生不受磷脂酶抑制剂的抑制，并没有开始加入磷脂酶A2的准备。因此，AA刺激NADPH氧化酶通过磷脂酶A2介导的途径在完整的细胞，但激活氧化酶独立的磷脂酶A2在一个破碎的细胞系统，这表明不同的激活机制，为每个系统。
The mechanism involved in the stimulation of NADPH-oxidase by arachidonic acid (AA) in intact human neutrophils was studied and compared with that involved in a cell-free system. [3H]-AA was released from pre-labeled cells upon AA stimulation, and phospholipase A2inhibitors reduced in parallel the release of [3H]-AA and superoxide. Cyclooxygenase, lipoxygenase or protein kinase inhibitors failed to affect either response. In a cell-free system, no release of [3H]-AA was observed after AA addition, whereas NADPH-oxidase was activated; the generation of superoxide was not inhibited by phospholipase inhibitors and was not initiated by adding phospholipase A2to the preparation. Thus AA stimulates NADPH-oxidase through a phospholipase A2mediated pathway in intact cells, but activates the oxidase independent of phospholipase A2in a broken cell system, suggesting distinctive mechanisms of activation for each system.