Moderate to severe water limitation differentially affects the phenome and ionome of Arabidopsis
Moderate to severe water limitation differentially affects the phenome and ionome of Arabidopsis
复制标题
DOI：
10.1071/fp16172
复制
发表时间：
2017-01-01
影响因子：
3
通讯作者：
Lorence, Argelia
中科院分区：
文献类型：
--
作者：
Acosta-Gamboa, Lucia M.;Liu, Suxing;Lorence, Argelia
粮食安全是目前人类面临的主要挑战之一。了解植物对有限水分供应的反应和适应是维持或提高作物产量的关键，考虑到对气候变化的不同预测，这一点就更加重要了。在这项工作中，我们结合了两个高通量组学平台（表型组学和离子组学），开始剖析拟南芥叶片水分限制的时间依赖性效应和最终的种子产量。作为概念验证，我们使用可见光、荧光和近红外相机获取了高分辨率图像，并使用商业和开源算法提取这些图像中包含的信息。在一个确定的点，样品也采取了元素分析。结果表明，在严格的水分限制制度下，生长、生物量和光合效率受到的影响最大，这些差异在发育后期加剧。然而，元素组成和种子产量在不同的水分条件下发生了变化，这些变化包括与水分充足的植物相比，元素积累不足和过量。我们的研究结果表明，表型技术的结合可以成功地用于识别植物发育过程中可能影响植物生物量、产量和营养质量的特定瓶颈。
Food security is currently one of the major challenges that we are facing as a species. Understanding plant responses and adaptations to limited water availability is key to maintain or improve crop yield, and this is even more critical considering the different projections of climate change. In this work, we combined two high-throughput -omic' platforms (phenomics' and ionomics') to begin dissecting time-dependent effects of water limitation in Arabidopsis leaves and ultimately seed yield. As proof of concept, we acquired high-resolution images with visible, fluorescence, and near infrared cameras and used commercial and open source algorithms to extract the information contained in those images. At a defined point, samples were also taken for elemental profiling. Our results show that growth, biomass and photosynthetic efficiency were affected mostly under severe water limitation regimes and these differences were exacerbated at later developmental stages. The elemental composition and seed yield, however, changed across the different water regimes tested and these changes included under- and over- accumulation of elements compared with well-watered plants. Our results demonstrate that the combination of phenotyping techniques can be successfully used to identify specific bottlenecks during plant development that could compromise biomass, yield, and the nutritional quality of plants.