THE PLASMID-ENCODED YOP2B PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS IS A VIRULENCE DETERMINANT REGULATED BY CALCIUM AND TEMPERATURE AT THE LEVEL OF TRANSCRIPTION
THE PLASMID-ENCODED YOP2B PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS IS A VIRULENCE DETERMINANT REGULATED BY CALCIUM AND TEMPERATURE AT THE LEVEL OF TRANSCRIPTION
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DOI：
10.1111/j.1365-2958.1988.tb00025.x
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发表时间：
1988-03-01
影响因子：
3.6
通讯作者：
WOLFWATZ, H
中科院分区：
文献类型：
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作者：
BOLIN, I;WOLFWATZ, H
基本的 Yop2b 蛋白由假结核耶尔森氏菌的毒力质粒 pIBI 编码，在 Ca2+ 缺乏的条件下产生。编码 Yop2b 蛋白的整个 yopH 基因被删除的突变体被发现是无毒的。毒力可以通过反式互补来恢复。 yopH 的 DNA 序列预测有 50 737 条多肽，缺乏典型的信号肽。 yopH 的转录受温度和 Ca2+ 浓度的调节。毒力质粒区域内的突变已知参与响应 Ca2+ 调节基因表达，从而消除了 yopH 的转录。 Ca2+ 调节位点中的其他温度敏感突变显示出高水平的转录，而与 Ca2+ 浓度无关。这些反应与 yopE 基因的反应相似。比较了 yopE 和 yopH 基因的启动子区域并鉴定了四个保守基序。
The basic Yop2b protein, encoded by the virulence plasmid pIBI of Yersinia pseudotuberculosis, is produced under Ca2+ -deficient conditions. A mutant deleted for the entire yopH gene, which encodes the Yop2b protein, was found to be avirulent. Virulence could be restored by trans-complementation. The DNA-sequence of yopH predicted a 50 737 polypeptide lacking a typical signal peptide. Transcription of yopH is regulated by both temperature and Ca2+-concentration. Mutations within the region of the virulence plasmid known to be involved in regulating gene expression in response to Ca2+ abolished transcription of yopH. Other temperature-sensitive mutations in the Ca2+-regulatory locus showed a high level of transcription regardless of Ca2+-concentration. These responses were similar to those of the yopE gene. The promoter region of the yopE and yopH genes were compared and four conserved motifs identified.