Effects of shear stress on endothelial cell haptotaxis on micropatterned surfaces
Effects of shear stress on endothelial cell haptotaxis on micropatterned surfaces
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DOI：
10.1016/j.bbrc.2005.08.272
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发表时间：
2005-11-11
影响因子：
3.1
通讯作者：
Li, S
中科院分区：
文献类型：
--
作者：
Hsu, S;Thakar, R;Li, S
血管内皮细胞(EC)迁移在血管重塑中起重要作用。在这里，我们研究了在EC迁移过程中，触觉(由细胞外基质梯度引起)和机械力(由机械力引起)之间的相互作用。利用微图案化技术产生胶原表面密度的阶跃变化。由于亲触性，内皮细胞形成局灶性粘连，并迁移到胶原表面密度较高的区域。在垂直于胶原条的方向上对内皮细胞施加不同程度的流体剪应力。2dyn/cm(2)的剪应力不影响内皮细胞的趋触性，而3dyn/cm(2)或更高的剪应力足以驱动大多数内皮细胞沿流动方向迁移，而不是亲和性。免疫组织化学染色显示局灶性粘连增多，板脂向血流方向突出。这些结果表明，超过一定阈值的剪应力可能是决定EC迁移方向的主要因素。(C)2005 Elsevier Inc.保留所有权利。
Endothelial cell (EC) migration plays a critical role in vascular remodeling. Here we investigated the interactions between haptotaxis (induced by extracellular matrix gradient) and mechanotaxis (induced by mechanical forces) during EC migration. A micropatterning technique was used to generate step changes of collagen surface density. Due to haptotaxis, ECs developed focal adhesions and migrated into the area with higher surface density of collagen. Different levels of fluid shear stress were applied on ECs in the direction perpendicular to collagen strips. Shear stress at 2 dyn/cm(2) did not affect haptotaxis, while shear stress at 3 dyn/cm(2) or higher was sufficient to drive the migration of most ECs in the flow direction and against haptotaxis. Immunostaining revealed the increase of focal adhesions and lamellipodial protrusion in the direction of flow. These results suggest that shear stress beyond a certain threshold can be a predominant factor to determine the direction of EC migration. (c) 2005 Elsevier Inc. All rights reserved.