The role of mast cell tryptase in neoangiogenesis of premalignant and malignant lesions of the uterine cervix
The role of mast cell tryptase in neoangiogenesis of premalignant and malignant lesions of the uterine cervix
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DOI：
10.1177/002215540104900816
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发表时间：
2001-08-01
影响因子：
3.2
通讯作者：
Castellanos, E
中科院分区：
文献类型：
--
作者：
Benítez-Bribiesca, L;Wong, A;Castellanos, E
最近，肥大细胞类胰蛋白酶已被确定为另一种有效的促血管生成因子在肿瘤中，沿着与成纤维细胞和血管内皮生长因子。它的作用已经在许多癌症中进行了研究，包括子宫颈癌，结果不一致。我们的目的是研究类胰蛋白酶和碱性成纤维细胞生长因子在肥大细胞（MCs）的表达在宫颈癌前病变和恶性病变的新生血管的发展。使用了21例非癌症患者和63例发育不良和鳞状细胞癌患者的活检标本。用阿新蓝-番红O（ABSO）染色，用抗因子VIII、CD 105、类胰蛋白酶和bFGF的特异性抗体进行免疫染色。胰蛋白酶阳性肥大细胞随着肿瘤进展而增加，并接近新形成的血管。从异型增生到浸润性癌，血管化呈线性增加。我们认为MC类胰蛋白酶可能上调子宫颈癌发生过程中的新生血管生成。
Recently, mast cell tryptase has been identified as another potent proangiogenic factor in tumors, along with fibroblast and vascular endothelial growth factors. Its role has been studied in a number of cancers, including carcinoma of the uterine cervix, with discordant results. Our aim was to study the expression of tryptase and bFGF in mast cells (MCs) during development of neoangiogenesis in premalignant and malignant lesions of the cervix. Biopsy specimens from 21 patients without cancer and from 63 patients with dysplasias and squamous cell carcinomas were used. They were stained with Alcian blue-safranin O (ABSO) and immunostained with specific antibodies against factor VIII, CD105, tryptase, and bFGF. Tryptase-positive mast cells increased with tumor progression and were close to newly formed blood vessels. Vascularization showed a linear increase from dysplasia to invasive cancer. We suggest that MC tryptase may upregulate neoangiogenesis in carcinogenesis of the uterine cervix.