A novel nucleic acid-binding protein that interacts with human Rad51 recombinase
A novel nucleic acid-binding protein that interacts with human Rad51 recombinase
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DOI：
10.1093/nar/25.24.4946
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发表时间：
1997-12-15
影响因子：
14.9
通讯作者：
Radding, CM
中科院分区：
文献类型：
--
作者：
Kovalenko, OV;Golub, EI;Radding, CM
利用酵母双杂交系统，我们分离出了编码一种新型人类蛋白质Pir51的cDNA，该蛋白质与人类Rad51重组酶强烈相互作用，体外分析证实了Rad51和Pir51之间的相互作用，Pir51 mRNA在许多人体器官中表达，最明显的是在睾丸、胸腺、结肠和小肠中，通过荧光将Pir51基因位点定位到染色体12p13.1-13.2原位杂交，Pir51 蛋白在大肠杆菌中表达并纯化至接近均质。生化分析表明，Pir51蛋白可结合单链和双链DNA，并且能够聚集DNA，该蛋白还结合RNA，Pir51蛋白可能代表多蛋白复合物的新成员，该复合物被假定在哺乳动物细胞中进行同源重组和DNA修复。
Using the yeast two-hybrid system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase, Analysis in vitro confirmed the interaction between Rad51 and Pir51, Pir51 mRNA is expressed in a number of human organs, most notably in testis, thymus, colon and small intestine, The Pir51 gene locus was mapped to chromosome 12p13.1-13.2 by fluorescence in situ hybridization, The Pir51 protein was expressed in Escherichia coli and purified to near homogeneity. Biochemical analysis shows that the Pir51 protein binds both single-and double-stranded DNA, and is capable of aggregating DNA, The protein also binds RNA, The Pir51 protein may represent a new member of the multiprotein complexes postulated to carry out homologous recombination and DNA repair in mammalian cells.