ATTACHMENT OF MITOCHONDRIA TO INTERMEDIATE FILAMENTS IN ADRENAL-CELLS - RELEVANCE TO THE REGULATION OF STEROID-SYNTHESIS
ATTACHMENT OF MITOCHONDRIA TO INTERMEDIATE FILAMENTS IN ADRENAL-CELLS - RELEVANCE TO THE REGULATION OF STEROID-SYNTHESIS
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DOI：
10.1016/0014-4827(92)90184-a
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发表时间：
1992-06-01
影响因子：
3.7
通讯作者：
HALL, PF
中科院分区：
文献类型：
--
作者：
ALMAHBOBI, G;WILLIAMS, LJ;HALL, PF
类固醇合成的速率受胆固醇从脂滴到线粒体的转运速率的调节。我们先前已经证明，肾上腺细胞中的脂滴紧密地附着在中间丝上。在这里，我们现在表明，线粒体与中间丝共定位在改良的双重间接免疫荧光和提取的肾上腺细胞的电子显微镜。线粒体和中间丝之间的直接接触，建立了检查立体对提取的细胞的电子显微照片。液滴和线粒体都附着在中间丝上，这表明这种胆固醇向线粒体的细胞内转运形式可能是机制，因此也可能是调节类固醇合成的机制。
The rate of steroid synthesis is regulated by the rate of transport of cholesterol from lipid droplets to mitochondria. We have previously demonstrated that lipid droplets in adrenal cells are tightly attached to intermediate filaments. Here we now show that mitochondria colocalize with intermediate filaments in modified double indirect immunofluorescence and by electron microscopy of extracted adrenal cells. Direct contact between mitochondria and intermediate filaments was established by examination of stereo pairs of electron micrographs from extracted cells. The attachment of both droplets and mitochondria to intermediate filaments suggests possible mechanisms for this form of intracellular transport of cholesterol to mitochondria and hence for the regulation of steroid synthesis.