Assessment of conjunctival flora in eyes with lacrimal passage obstruction before and after successful dacryoendoscopic recanalisation
Assessment of conjunctival flora in eyes with lacrimal passage obstruction before and after successful dacryoendoscopic recanalisation
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DOI：
10.1136/bjophthalmol-2020-316264
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发表时间：
2021-07-01
影响因子：
4.1
通讯作者：
Oshika, Tetsuro
中科院分区：
文献类型：
--
作者：
Hiraoka, Takahiro;Hoshi, Sujin;Oshika, Tetsuro
目的探讨泪道阻塞患者泪道内窥镜下泪道再通术前后结膜植物群的变化。方法回顾性分析150例泪道阻塞患者的临床资料。在手术前和手术后4个月对每只眼睛进行结膜取样。用无菌棉拭子擦拭下穹窿，并将收集的样品用几个琼脂平板培养。通过标准细菌学实验室技术对菌落进行区分和计数。结果术前细菌培养阳性率为42.0%，术后细菌培养阳性率为26.0%，差异有统计学意义（P=0.0051）。检出菌株数也由术前的20株减少到术后的9株，尤其是致病微生物减少。此外，术前在9只眼睛中检测到耐青霉素肺炎链球菌和耐甲氧西林金黄色葡萄球菌等耐药细菌，但术后仅在1只眼睛中检测到。凝固酶阴性葡萄球菌和棒状杆菌属，正常结膜植物群中常见的细菌，术前占46.5%，术后占80.9%，术后明显增加（P
Background To investigate conjunctival bacterial flora in eyes with lacrimal passage obstruction before and after dacryoendoscopic recanalisation with lacrimal tube intubation. Methods One-hundred fifty eyes with lacrimal passage obstruction that were successfully treated by dacryoendoscopic recanalisation were enrolled. Conjunctival sampling was done for each eye before and 4 months after surgery. The lower fornix was rubbed by a sterile cotton swab, and the collected samples were cultured with several agar plates. Colonies were differentiated and enumerated by standard bacteriological laboratory techniques. Results Positive bacterial growth was detected in 42.0% of all the samples before surgery, and the positivity rate significantly decreased to 26.0% after surgery (p=0.0051). The number of strains detected also decreased from 20 before surgery to 9 after surgery, especially pathogenic microorganisms decreased. In addition, drug-resistant bacteria such as penicillin-resistant Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus were detected in nine eyes before surgery but were detected only in one eye after surgery. Coagulase-negative Staphylococci and Corynebacterium spp., which are common in normal conjunctival flora, accounted for 46.5% of all the isolates before surgery and 80.9% after surgery, showing a significant increase in the rate after surgery (p