Correlation between viscosity and sialic acid content of whole human saliva.
Correlation between viscosity and sialic acid content of whole human saliva.
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人类全唾液粘度与唾液酸含量之间的相关性。
DOI：
10.2329/perio.22.366
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
L. S. Chen
中科院分区：
文献类型：
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作者：
F. Ito;S. Yamada;Y. Mizuno;N. Sugihara;L. S. Chen
本研究观察了人全唾液粘度和唾液酸含量随时间的变化。从7名临床健康的男性（年龄24至34岁）获得全唾液样品，两天（48小时）没有任何口腔清洁。在两餐之间收集刷牙5分钟前后的唾液，并立即冷却至0 ℃以下。对部分唾液立即进行以下测试，对其余唾液在1、2、4、6小时的孵育期（37°C）后进行以下测试。以厘泊为单位的粘度（c.第页）在37°C下使用ELD型锥板粘度计以76.80sec-1的剪切速率测量。以唾液酸为标准品，采用硫代巴比妥酸法测定唾液酸。在37°C孵育期间，唾液粘度和唾液酸含量均显示出时间依赖性降低。在刷牙前收集的唾液中观察到这两项指标的下降更快。两组患者术后2小时内唾液粘度的下降差异无统计学意义。但在刷牙前收集的唾液中，经过6小时的潜伏期后，下降速度更快。刷牙前后唾液中总唾液酸和结合唾液酸含量在各时间段差异均有统计学意义.两组唾液中游离唾液酸含量仅在唾液采集后即刻有统计学差异.唾液粘度与结合唾液酸含量之间仅在采集后即刻存在统计学上的显著正相关.用同样的测定条件孵育6小时后，无菌全唾液中唾液粘度和结合唾液酸均未降低，唾液酸降解与粘度降低密切相关，推测这些现象是由唾液神经氨酸酶和醛缩酶活性引起的。
In this study, time-dependent change of whole human salivary viscosity and sialic acid content were investigated. Whole saliva samples were obtained from seven clinically healthy human males (aged 24 to 34 years) without any mouth cleaning for two days (48 hours). The saliva before and after 5min-brushing was collected between meals and immediately cooled down to below 0°C. The following tests were immediately for a part of the saliva and after 1, 2, 4, 6 hours of incubation period (37°C) for the rest of the saliva. The viscosity in Centipoises (c. p.) was measured at 37°C using model ELD cone-plate viscometer, with a shear rate of 76.80sec-1. Sialic acid was measured by the thiobarbituric acid assay using sialic acid as standard.The following results were obtained:1. Both salivary viscosity and sialic acid content showed a time-dependent decrease during incubation at 37°C. A more rapid decrease of both measures was observed in the saliva collected before brushing.2. There was no significant difference in the decrement of salivary viscosity between both groups within 2 hours. But a more rapid decrease was found in the saliva collected before brushing after 6 hours of incubation period.3. There were obtained statistically significant differences in the total and bound sialic acid content between the saliva before and after brushing at every time interval.4. A statistically significant difference was found in the free sialic acid content between the two groups only immediately after collection of saliva.5. A statistically significant positive correlation was found between the salivary viscosity and the bound sialic acid content only immediately after collection.6. Neither a decrease of salivary viscosity nor a degradation of bound sialic acid were obtained in sterilized whole saliva even after 6 hours incubation using the same assay condition.It was concluded that sialic acid degradation are closely related to the decrease in viscosity and presumably these phenomena are caused by salivary neuraminidase and aldolase activity.