Campylobacter jejuni capsular genotypes are related to Guillain-Barre syndrome
Campylobacter jejuni capsular genotypes are related to Guillain-Barre syndrome
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DOI：
10.1016/j.cmi.2015.05.031
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发表时间：
2015-09-01
影响因子：
14.2
通讯作者：
Endtz, H. P.
中科院分区：
文献类型：
--
作者：
Heikema, A. P.;Islam, Z.;Endtz, H. P.
在大约千分之一的病例中，空肠弯曲杆菌感染导致严重的多发性神经病格林-巴利综合征（GBS）。研究表明，C.空肠是GBS发展中关键毒力因子。C.在来自无并发症肠炎患者的粪便中的空肠中存在唾液酸化LOS意味着应该涉及额外的细菌因素。为了评估多糖荚膜是否是GBS的标志物，对两种地理上不同的GBS相关的C。确定空肠菌株收集物和无并发症肠炎对照收集物。C.来自荷兰的空肠菌株显示，与单纯性肠炎相比，三种荚膜基因型，HS 1/44 c、HS 2和HS 4c，在GBS相关菌株中占优势，并且荚膜类型HS 1/44 c和HS 4c与GBS显著相关（分别为p 0.05和p 0.01）。在来自孟加拉国的GBS相关菌株采集中，荚膜类型HS 23/36 c、HS 19和HS 41最常见，荚膜类型HS 19和HS 41与GBS相关（分别为p 0.008和p 0.02）。接下来，确定了与GBS发生相关的LOS类别和荚膜基因型的特定组合。多位点序列分型显示，有限的遗传多样性与荚膜类型HS 2，HS 19和HS 41的菌株群体。我们得出结论，荚膜类型HS 1/44 c，HS 2，HS 4c，HS 19，HS 23/36 c和HS 41是GBS的标志物。除了C.空肠在GBS发病机制中的作用，确定的胶囊可能有助于GBS的易感性。临床微生物学和感染（C）2015年欧洲临床微生物学和传染病学会。由爱思唯尔有限公司出版。保留所有权利。
In about one in a thousand cases, a Campylobacter jejuni infection results in the severe polyneuropathy Guillain-Barre syndrome (GBS). It is established that sialylated lipo-oligosaccharides (LOS) of C. jejuni are a crucial virulence factor in GBS development. Frequent detection of C. jejuni with sialylated LOS in stools derived from patients with uncomplicated enteritis implies that additional bacterial factors should be involved. To assess whether the polysaccharide capsule is a marker for GBS, the capsular genotypes of two geographically distinct GBS-associated C. jejuni strain collections and an uncomplicated enteritis control collection were determined. Capsular genotyping of C. jejuni strains from the Netherlands revealed that three capsular genotypes, HS1/44c, HS2 and HS4c, were dominant in GBS-associated strains and capsular types HS1/44c and HS4c were significantly associated with GBS (p 0.05 and p 0.01, respectively) when compared with uncomplicated enteritis. In a GBS-associated strain collection from Bangladesh, capsular types HS23/36c, HS19 and HS41 were most prevalent and the capsular types HS19 and HS41 were associated with GBS (p 0.008 and p 0.02, respectively). Next, specific combinations of the LOS class and capsular genotypes were identified that were related to the occurrence of GBS. Multilocus sequence typing revealed restricted genetic diversity for strain populations with the capsular types HS2, HS19 and HS41. We conclude that capsular types HS1/44c, HS2, HS4c, HS19, HS23/36c and HS41 are markers for GBS. Besides a crucial role for sialylated LOS of C. jejuni in GBS pathogenesis, the identified capsules may contribute to GBS susceptibility. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.