Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells
Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells
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DOI：
10.1039/b705085m
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发表时间：
2007-06-01
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Karp, Jeffrey M.;Yeh, Judy;Khademhosseini, Ali
胚胎干细胞（ES）的定向分化可用于建立人类疾病模型，并可能产生广泛的功能细胞类型用于治疗应用。分化ES细胞的方法通常涉及形成称为胚状体（EB）的细胞聚集体，其重现胚胎发育的早期阶段。EB通常由悬浮培养物制成，从而产生具有广泛尺寸和形状的异质结构，这可能会影响分化。在这里，我们使用微制造的细胞排斥性聚乙二醇（PEG）威尔斯作为模板，以启动均匀的EB的形成。ES细胞聚集体形成为具有由微孔的几何形状限定的受控尺寸和形状。以这种方式产生的EB保持活力，并相对于其悬浮液对应物在微孔内保持其大小和形状。完整的EB可以很容易地从微孔中回收，具有高活力（> 95%）。这些结果表明，微孔技术可能是一个有用的方法，在体外研究涉及ES细胞，更具体地说，用于启动分化的EB的更大的均匀性的基础上控制的微环境。
Directed differentiation of embryonic stem (ES) cells is useful for creating models of human disease and could potentially generate a wide array of functional cell types for therapeutic applications. Methods to differentiate ES cells often involve the formation of cell aggregates called embryoid bodies (EBs), which recapitulate early stages of embryonic development. EBs are typically made from suspension cultures, resulting in heterogeneous structures with a wide range of sizes and shapes, which may influence differentiation. Here, we use microfabricated cell-repellant poly(ethylene glycol) (PEG) wells as templates to initiate the formation of homogenous EBs. ES cell aggregates were formed with controlled sizes and shapes defined by the geometry of the microwells. EBs generated in this manner remained viable and maintained their size and shape within the microwells relative to their suspension counterparts. Intact EBs could be easily retrieved from the microwells with high viability (> 95%). These results suggest that the microwell technique could be a useful approach for in vitro studies involving ES cells and, more specifically, for initiating the differentiation of EBs of greater uniformity based on controlled microenvironments.