The histone acetyltransferase p300/CBP-associated factor acts as an effective suppressor of secretory immunoglobulin synthesis in immature B cells
The histone acetyltransferase p300/CBP-associated factor acts as an effective suppressor of secretory immunoglobulin synthesis in immature B cells
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组蛋白乙酰转移酶 p300/CBP 相关因子是未成熟 B 细胞中分泌性免疫球蛋白合成的有效抑制剂
DOI：
10.1111/1348-0421.12237
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Tatsuo Nakayama
中科院分区：
文献类型：
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作者：
Hidehiko Kikuchi;Masami Nakayama;Chikage Kawai;Futoshi Kuribayashi;Hitomi Mimuro;Shinobu Imajoh-Ohmi;Nishitoh H;Yasunari Takami;and Tatsuo Nakayama
组蛋白乙酰转移酶p300/CBP相关因子（PCAF）催化核心组蛋白的乙酰化，并通过改变脊椎动物的染色质结构在表观遗传学中发挥重要作用。在这项研究中，分析了PCAF缺陷型DT 40突变体，发现PCAF参与调节分泌型IgM重链（H-链）的合成。值得注意的是，PCAF缺乏导致分泌型IgM H链mRNA的量增加，但IgM轻链和膜结合型IgM H链mRNA的量不增加，导致分泌型IgM蛋白的量显著上调。这些发现表明PCAF调节可溶性抗体的产生，因此是分泌型IgM H链合成的有效抑制剂。
The histone acetyltransferase p300/CBP‐associated factor (PCAF) catalyzes acetylation of core histones and plays important roles in epigenetics by altering the chromatin structure in vertebrates. In this study, PCAF‐deficient DT40 mutants were analyzed and it was found that PCAF participates in regulation of secretory IgM heavy chain (H‐chain) synthesis. Remarkably, PCAF‐deficiency causes an increase in the amount of secretory IgM H‐chain mRNA, but not in that of IgM light chain and membrane‐bound IgM H‐chain mRNAs, resulting in dramatic up‐regulation of the amount of secretory IgM protein. These findings suggest that PCAF regulates soluble antibody production and is thus an effective suppressor of secretory IgM H‐chain synthesis.