Overexpression of AtHsfB4 induces specific effects on root development of Arabidopsis
Overexpression of AtHsfB4 induces specific effects on root development of Arabidopsis
复制标题
DOI：
10.1016/j.mod.2012.05.008
复制
发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Schoeffl, Friedrich
中科院分区：
文献类型：
--
作者：
Begum, Tahmina;Reuter, Rolf;Schoeffl, Friedrich
植物b类热休克因子（Hsfs）的功能尚不清楚。属于这一类的Hsfs与a类Hsfs的不同之处在于其寡聚化结构域的结构特征以及缺乏用于转录激活的典型AHA基序。AtHsfB4在植物的不同部位均有表达，在根组织中表达量最高。camv - 35s启动子过表达（OE） HsfB4的转基因拟南芥植株显示Hsf mrna水平大幅提高。e -植物根表面粗糙，细胞脱落。利用细胞类型特异性根标记系和共聚焦激光扫描显微镜进行杂交，明确了HsfB4-OE植株地面组织和侧根帽（LRC）细胞异位层细胞复制的证据。胚胎发育过程中已经发生了内胚层细胞的复制，而异位LRC细胞仅在胚胎后生长过程中被检测到。Hsf-OE植株的突变表型没有先例，表明b类hsf可能在根系发育中起重要作用。2012爱思唯尔爱尔兰有限公司版权所有。
The functions of plant class B-heat shock factors (Hsfs) are not well understood. Hsfs belonging to this group differ from class A-Hsfs in structural features of the oligomerization domain and by the absence of a typical AHA motif for transcriptional activation. AtHsfB4 is expressed in different parts of the plants with highest levels in root tissue. Transgenic Arabidopsis plants overexpressing (OE) HsfB4 by CaMV-35S-promoter showed massively enhanced levels of Hsf mRNAs. The root surface of OE-plants was rough and cells became detached. Crossings with cell type specific root marker lines and confocal laser scanning microscopy provided clear evidence for a duplication of cells in the ground tissue and ectopic layers of lateral root cap (LRC) cells in HsfB4-OE plants. A duplication of endodermis cells occurs already during embryonic development, while the ectopic LRC cells are only detected during postembryonic growth. The mutant phenotypes of Hsf-OE plants are without precedence and indicate that class B-Hsfs may play an important role in root development. (c) 2012 Elsevier Ireland Ltd. All rights reserved.