NUCLEOTIDE-SEQUENCE OF TOBACCO MOSAIC-VIRUS RNA
NUCLEOTIDE-SEQUENCE OF TOBACCO MOSAIC-VIRUS RNA
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DOI：
10.1073/pnas.79.19.5818
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
KARN, J
中科院分区：
文献类型：
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作者：
GOELET, P;LOMONOSSOFF, GP;KARN, J
使用寡核苷酸引物生成覆盖整个烟草花叶病毒（TMV）RNA序列的c[互补]DNA文库。对这些克隆的分析可以完成病毒 RNA 序列并研究其在病毒群体中的变异性。正链编码序列从 5'' 端开始 69 个核苷酸，具有来自 MW 125,941 蛋白质的阅读框，并以 UAG 终止。通读该终止子将产生分子量为 183,253 的蛋白质。与该通读框的末端 5 个密码子重叠的是编码分子量为 29,987 的蛋白质的第二阅读框。该基因在外壳蛋白基因的起始密码子之前终止2个核苷酸。鉴定了负责外壳蛋白和 MW 29,987 蛋白 mRNA 的加帽和合成的潜在信号序列。这些阅读框中的相似序列可用于表达共享COOH末端序列的蛋白质组。
Oligonucleotide primers were used to generate a c[complementary]DNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones permitted completion of the viral RNA sequence and study of its variability within a viral population. The positive strand coding sequence starts 69 nucleotides from the 5'' end with a reading frame from a protein of MW 125,941 and terminates with UAG. Readthrough of this terminator would give rise to a protein of MW 183,253. Overlapping the terminal 5 codons of this readthrough frame is a 2nd reading frame coding for a protein of MW 29,987. This gene terminates 2 nucleotides before the initiator codon of the coat protein gene. Potential signal sequence responsible for the capping and synthesis of the coat protein and MW 29,987 protein mRNA were identified. Similar sequences within these reading frames may be used in the expression of sets of proteins that share COOH-terminal sequences.