Alterations of dendritic protrusions over the first postnatal year of a mouse: an analysis in layer VI of the barrel cortex.
Alterations of dendritic protrusions over the first postnatal year of a mouse: an analysis in layer VI of the barrel cortex.
复制标题
小鼠出生后第一年树突突起的变化：桶状皮质第六层的分析。
DOI：
10.1007/s00429-013-0596-5
复制
发表时间：
2014
影响因子：
3.1
通讯作者：
Brumberg,JoshuaC
中科院分区：
文献类型：
--
作者：
Orner,DavidA;Chen,Chia-Chien;Orner,DaniellaE;Brumberg,JoshuaC
树突棘是小的突起，作为兴奋性输入到皮质锥体细胞的主要接受者。棘和丝状伪足密度和形态的改变与发育成熟和突触强度的变化相关。为了更好地了解树突突起（树突棘+丝状伪足）形态和密度在动物出生后第一年的发育概况，我们使用高尔基体染色技术标记小鼠神经元及其树突突起。我们专注于量化单位长度的树突的密度和分类的形态树突突起的第VI层锥体神经元居住在桶皮质使用计算机辅助重建程序Neurolucida。我们分类树突突起密度在7个发育时间点：出生后（PND）15，30，60，90，180，270，和360。我们的研究结果表明，第VI层桶皮质锥体神经元的树突突起不是静态的，其密度以及相对形态分布随时间而变化。我们观察到一个显着增加蘑菇刺和减少丝状伪足的动物成熟。进一步的分析表明，随着动物的成熟，锥体细胞树突长度总体上减少，以及总体突起密度减少。顶基底密度比值也降低。在出生后的第一年内表征皮质层VI树突状突起的轮廓将使我们能够更好地了解整体发育成熟轮廓和树突棘功能之间的关系。
Dendritic spines are small protrusions that serve as the principal recipients of excitatory inputs onto cortical pyramidal cells. Alterations in spine and filopodia density and morphology correlate with both developmental maturity and changes in synaptic strength. In order to better understand the developmental profile of dendritic protrusion (dendritic spines + filopodia) morphology and density over the animal’s first postnatal year, we used the Golgi staining technique to label neurons and their dendritic protrusions in mice. We focused on quantifying the density per length of dendrite and categorizing the morphology of dendritic protrusions of layer VI pyramidal neurons residing in barrel cortex using the computer assisted reconstruction program Neurolucida. We classified dendritic protrusion densities at seven developmental time points: postnatal day (PND) 15, 30, 60, 90, 180, 270, and 360. Our findings suggest that the dendritic protrusions in layer VI barrel cortex pyramidal neurons are not static, and their density as well as relative morphological distribution change over time. We observed a significant increase in mushroom spines and a decrease in filopodia as the animals matured. Further analyses show that as the animal mature there was a reduction in pyramidal cell dendritic lengths overall, as well as a decrease in overall protrusion densities. The ratio of apical to basilar density decreased as well. Characterizing the profile of cortical layer VI dendritic protrusions within the first postnatal year will enable us to better understand the relationship between the overall developmental maturation profile and dendritic spine functioning.