Thymol and Thymus Vulgaris L. activity against UVA- and UVB-induced damage in NCTC 2544 cell line
Thymol and Thymus Vulgaris L. activity against UVA- and UVB-induced damage in NCTC 2544 cell line
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DOI：
10.1016/j.mrgentox.2015.07.009
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发表时间：
2015-09-01
影响因子：
1.9
通讯作者：
Marabini, Laura
中科院分区：
文献类型：
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作者：
Calo, Rossella;Visone, Clementina M.;Marabini, Laura
许多作者专注于天然化合物的研究，以保护皮肤免受间接（UVA）和直接（UVB）紫外线辐射的副作用。本研究的目的是评估 T. vulgaris L. 的干提取物及其主要合成化合物百里香酚（约 60%）对氧化和基因毒性 UVA 和 UVB 损伤的保护作用。实验在低分化角质形成细胞系 (NCTC 2544) 中重复进行。细胞在无血清培养基中用百里香酚 (1 μg/mL) 或 T. vulgaris L. (1.82 μg/mL) 预处理 1 小时，然后暴露于不同的 UVA (8-24 J/cm(2)) 或 UVB 剂量 (0.016-0.72 J/cm(2))。紫外线照射后立即通过 ROS 定量和 LPO 评估细胞内氧化还原状态。照射结束后 24 小时，使用碱性彗星测定、微核形成测定和磷酸化 H2AX 组蛋白的免疫染色（在紫外线照射结束后 1 小时检测）评估基因毒性方面。百里酚和 T. vulgaris L. 提取物抑制 UVA 和 UVB 照射的细胞中 ROS 的产生。相反，MDA 形成仅在 UVA 处理的细胞中减少。两种药物均减少了碱性彗星试验评估的 DNA 损伤，但在微核和 H2AX 测试中却没有减少，可能是因为检测到损伤（双链）的严重程度。 (C) 2015 Elsevier B.V. 保留所有权利。
Many authors focused on the research of natural compounds in order to protect skin from indirect (UVA) and direct (UVB) ultraviolet radiation side effects. The aim of this study to evaluate the protective effect of a dry extract from T. vulgaris L. and of its major synthetic compound thymol (about 60%), against oxidative and genotoxic UVA- and UVB damage. Experiments were reproduced in a low differentiated keratinocytes cell line (NCTC 2544) Cells were pretreated for 1 h, in serum-free medium, with thymol (1 mu g/mL) or T. vulgaris L. (1.82 mu g/mL) then exposed to different UVA (8-24 J/cm(2)) or UVB doses (0.016-0.72 J/cm(2)). Immediately after the UV exposure the intracellular redox status was evaluated by ROS quantification and by LPO. Genotoxic aspects were evaluated 24h after the end of irradiations using the alkaline comet assay, the micronucleus formation assay and the immunostaining of phosphorylated H2AX histone protein (detected 1 h after the end of UV exposure). Thymol and T. vulgaris L. extract inhibited ROS generation in UVA and UVB-irradiated cells. On the contrary, MDA formation was reduced only in UVA treated cells. Both agents decreased the DNA damage evaluated by the alkaline comet assay, but not in the micronucleus and H2AX tests probably because of the severity of damage (double strands) detected. (C) 2015 Elsevier B.V. All rights reserved.