A biochemical sniffer-chip for convenient analysis of gaseous formaldehyde from timber materials
A biochemical sniffer-chip for convenient analysis of gaseous formaldehyde from timber materials
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DOI：
10.1007/s00604-007-0799-7
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发表时间：
2008-04
影响因子：
5.7
通讯作者：
K. Mitsubayashi;G. Nishio;M. Sawai;Elito Kazawa;Hiromichi Yoshida;Takao Saito;H. Kudo;K. Otsuka-K.-Otsuk
中科院分区：
文献类型：
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作者：
K. Mitsubayashi;G. Nishio;M. Sawai;Elito Kazawa;Hiromichi Yoshida;Takao Saito;H. Kudo;K. Otsuka-K.-Otsuk
研制了一种甲醛脱氢酶（HMDH）生物化学气敏传感器（嗅探芯片），用于对气态甲醛的高选择性分析。通过将α-羟甲基脱氢酶固定在涂有铂电极的亲水性聚四氟乙烯膜上，构建了气相中甲醛的嗅探芯片。通过安培分析测量由酶促反应产生的NADH的氧化电流。该芯片对气相中甲醛的校准范围为40 ~ 2000 ppb，满足住宅中甲醛蒸气的最大允许浓度（80 ppb）和人类嗅觉甲醛检测限（410 ppb）。作为住宅环境应用，该嗅探器芯片可以在3 min内测量一些木材材料（3种室内木材和2种外模板木材）中的甲醛浓度。通过回归分析计算的浓度值与市售气体传感器和甲醛气体检测管的浓度值一致。该吸头具有气体选择性高的特点，是检测和测量室内空气中甲醛气体的一种简便有效的方法。
A biochemical gas-sensor (sniffer-chip) with formaldehyde dehydrogenase (FALDH) was developed for convenient analysis of gaseous formaldehyde with high gas-selectivity. The sniffer-chip for formaldehyde in the gas phase was constructed by immobilizing FALDH to a Pt-electrode coated hydrophilic PTFE membrane. The oxidation current of NADH produced by the enzymatic reactions was measured by amperometric analysis. The calibration range of the sniffer-chip for formaldehyde in the gas phase was from 40 to 2000 ppb, which encountered the maximum permissible concentration of formaldehyde vapor in the residential house (80 ppb) and formaldehyde detection limit for the human sense of smell (410 ppb). As the resident-environmental application, the sniffer-chip was possible to measure the formaldehyde concentration from some timber materials (3 kinds of interior timber and 2 kinds of exterior formwork timber) within 3 min. The calculated concentration value by a regression analysis was consistent with those of a commercially available gas sensor and a gas detector tube for formaldehyde. The FALDH sniffer-tip would be effective and convenient approach to detect and measure gaseous formaldehyde with high gas-selectivity at the residential atmosphere.