THE ZYGOSPORE WALL OF CHLAMYDOMONAS MONOICA (CHLOROPHYCEAE): MORPHOGENESIS AND EVIDENCE FOR THE PRESENCE OF SPOROPOLLENIN 1
THE ZYGOSPORE WALL OF CHLAMYDOMONAS MONOICA (CHLOROPHYCEAE): MORPHOGENESIS AND EVIDENCE FOR THE PRESENCE OF SPOROPOLLENIN 1
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衣藻（绿藻科）的接合孢子壁：形态发生和孢粉素 1 存在的证据
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
W. Rickoll
中科院分区：
文献类型：
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作者：
K. Vanwinkle‐swift;W. Rickoll
单衣单胞菌Strehlow正在被开发作为合子形态发生的遗传分析模型，并且有许多相关的突变菌株可用。为了为解释合子孢子突变体的超微结构表型提供依据，本文对野生型单角霉合子孢子的细胞壁形态发生进行了分析。随着厚的纤维状初生合子壁的合成，颗粒状物质在质膜和初生合子壁之间积累，聚集成一组重复的电子不透明纤维条纹。一个新的壁层，即次级接合壁的外层，首先以纤维状的外表面覆盖在一个明确的电子半透明材料带上的片段出现。这些节段在质膜附近形成完整的鞘。在鞘层下面，电子不透明的物质（形成次级合子壁的内层）不均匀地积累，迫使表面鞘层波动，形成一个波峰和波谷的图案，暴露在外部环境中，导致初级合子壁破裂和释放。接合核壁包括抗氢氧化钾、2 -氨基乙醇和乙酰解降解的材料，但暴露在铬酸中会被破坏。这些特征，再加上未经处理的合子细胞壁的自身荧光，以及它们不能被间苯三酚染色，表明孢子孢粉可能是衣藻成熟合子的许多抗性特性的原因。
Chlamydomonas monoica Strehlow is being developed as a model for genetic analysis of zygospore morphogenesis, and many relevant mutant strains are available. To provide the basis for interpreting the ultrastructural phenotypes of zygospore mutants, an analysis of wall morphogenesis in wildtype zygospores of C. monoica was undertaken. Following synthesis of a thick, fibrous, primary zygote wall, granular material accumulated between the plasma membrane and the primary zygote wall and aggregated into a repetitive array of electron‐opaque fibrous stripes. A new wall layer, the outer layer of the secondary zygospore wall, first appeared as segments with a fibrous outer surface overlying a well‐defined band of electron‐translucent material. These segments gave rise to an intact sheath adjacent to the plasma membrane. Beneath this sheath, electron‐opaque material (forming the inner layer of the secondary zygospore wall) accumulated unevenly and forced the surface sheath to undulate, creating a pattern of peaks and valleys that was exposed to the external environment 4 rupture and release of the primary zygote wall. The zygospore wall included material resistant to degradation by potassium hydroxide, 2‐aminoethanol, and acetolysis, but it was destroyed by exposure to chromic acid. These characteristics, in combination with the autofluorescence of untreated zygospore walls and their failure to stain with phloroglucinol, suggest that sporopollenin may be responsible for many of the resistant properties associated with the mature zygospore of Chlamydomonas.