Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry
Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry
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DOI：
10.1039/c1ja10227c
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发表时间：
2011-01-01
影响因子：
3.4
通讯作者：
Jakubowski, Norbert
中科院分区：
文献类型：
--
作者：
Giesen, Charlotte;Waentig, Larissa;Jakubowski, Norbert
建立了一种新的激光消融-ICP-MS单细胞及细胞核成像方法。其中，碘被用作成纤维细胞和薄组织切片的元素染料。在60 s的孵育时间内，碘主要位于细胞核内。这种效应在成纤维细胞中得到证实，在4 μ m激光光斑尺寸下，细胞核内的碘信号高达5 x 10(4) cps。周围的细胞质也被碘化，但程度较轻。获得的空间分辨率足以检测肝活检组织中更小的细胞核。此外，碘被成功地用于生物分子标记，我们证明了碘信号随着腭扁桃体组织厚度的增加而增加。因此，研究了在LA-ICP-MS中使用碘作为内标来纠正组织不均匀性，以同时检测乳腺癌组织中的两种肿瘤标志物（Her 2和CK 7）。此外，由玻璃烧蚀引起的镧系本底可以通过Eu标准化进行校正。
A new laser ablation (LA)-ICP-MS method for single cell and cell nucleus imaging was developed. Therein, iodine was employed as an elemental dye for fibroblast cells and for thin tissue sections. At an incubation time of 60 s, iodine is located mainly within the cell nuclei. This effect was illustrated in fibroblast cells, and iodine signal within the cell nucleus was as high as 5 x 10(4) cps at 4 mu m laser spot size. The surrounding cytoplasm was iodinated as well, but to a lesser extent. The spatial resolution attained was sufficient to detect even smaller cell nuclei within a liver biopsy tissue. Furthermore, iodine was successfully employed for biomolecule labeling and we demonstrated that iodine signal increased with increasing thickness of a palatine tonsil tissue. Thus, the use of iodine as an internal standard to correct for tissue inhomogeneities in LA-ICP-MS was investigated for the simultaneous detection of two tumor markers (Her 2 and CK 7) in breast cancer tissue. Additionally, lanthanide background resulting from glass ablation can be corrected for by Eu standardization.