STUDIES OF ANTIGENIC DIFFERENCES AMONG STRAINS OF INFLUENZA A BY MEANS OF RED CELL AGGLUTINATION.
STUDIES OF ANTIGENIC DIFFERENCES AMONG STRAINS OF INFLUENZA A BY MEANS OF RED CELL AGGLUTINATION.
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DOI：
10.1084/jem.78.5.407
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发表时间：
1943-11-01
期刊：
影响因子：
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通讯作者：
Hirst GK
中科院分区：
文献类型：
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作者：
Hirst GK
对甲型流感病毒株及其抗血清之间的交叉抑制试验的研究表明，由于所用的红细胞、病毒悬液和雪貂抗血清，所得结果会有一定的变异。已经证明了处理从这些试验中获得的数据的方法，以便纠正或避免这些变量，从而可以使用凝集技术进行抗原比较。用雪貂抗血清进行凝集抑制试验，比较了1940- 1941年美国流行的18株A型流感病毒的抗原模式。在这些菌株中，尽管它们是从该国广泛分离的地区的个体中获得的，但在16个菌株（均通过接种鸡胚从咽喉冲洗液中分离）中未发现显著的抗原差异。两个菌株，从发生在流行病早期的病例中分离出来，通过雪貂和小鼠传代从咽喉冲洗液中分离出来，与其他菌株以及彼此之间显示出轻微但显著的菌株差异。在交叉试验中，1940-41菌株中的一个与PR 8菌株的相似性比任何其他试验的原种菌株都要高。
A study of cross inhibition tests among strains of influenza A virus and their antisera showed that the results obtained were subject to a certain amount of variation due to the red cells, the virus suspensions, and the ferret antisera employed. Methods have been demonstrated for handling the data obtained from such tests, so that these variables were corrected or avoided, making it possible to use the agglutination technique for antigenic comparisons. The antigenic pattern of eighteen strains of influenza A virus, obtained from the 1940–41 epidemic in the United States, has been compared by means of agglutination inhibition tests with ferret antisera. No significant antigenic differences were found among sixteen of these strains (all isolated from throat washings by the inoculation of chick embryos) although they were obtained from individuals in widely separated regions of the country. Two strains, from cases occurring early in the epidemic and isolated from throat washings by ferret and mouse passage, showed a slight but significant strain difference from the other strains and from each other. One of the 1940–41 strains on cross test resembled the PR8 strain more closely than any other stock strain tested.