ASEXUAL REPRODUCTION AND BEHAVIOR OF ZOOSPORES OF PHYTOPHTHORA MEGASPERMA VAR. SOJAE
ASEXUAL REPRODUCTION AND BEHAVIOR OF ZOOSPORES OF PHYTOPHTHORA MEGASPERMA VAR. SOJAE
复制标题
疫霉菌大孢子的无性繁殖和游动孢子的行为。
作者：
H. Ho;C. Hickman
研究了一种在蒸馏水悬浮液中从巨型疫霉纯培养物中产生大量游动孢子的方法。烧酒。孢子囊主要无乳突，但偶尔不明显或明显乳突，萌发时形成一个精致的、瞬息万变的小泡。观察到孢子囊的增殖，并对游动孢子自由游动的弗拉格拉作用进行了摄影观察。两根鞭毛均呈波状分布。游动孢子在15℃下活动时间最长，在极端温度(5℃和36℃)、极端pH值(5.2以下或9.25以上)、机械干扰、稀释和频繁接触固体表面时，游动孢子的活力显著降低。观察包囊过程中鞭毛的去向。包囊游动孢子由芽管萌发，或由次生游动孢子萌发，或在极少数情况下，芽管由一个微型孢子囊终止。15℃有利于游动孢子的反复萌发，而孢子萌发总量和生殖管产量较低。
A method was devised to produce abundant zoospores in distilled water suspension from pure cultures of Phytophthora megasperma var. sojae. Sporangia were predominantly non-papillate, but occasionally inconspicuously or conspicuously papillate, germinating with the formation of a delicate, evanescent vesicle. Proliferation of sporangia was observed.Flagella action of freely swimming zoospores was investigated photographically. Both flagella were proved to undulate. Zoospores remained motile longest at 15 °C. Motility was markedly reduced at extreme temperatures (5 and 36 °C), at extreme pH values (below 5.2 or above 9.25), or by mechanical disturbance, dilution, and frequent contact with solid surfaces. The fate of flagella during encystment was followed. Encysted zoospores germinated by germ tubes, or by secondary zoospores, or in rare cases, the germ tube was terminated by a miniature sporangium. Repeated emergence of zoospores was favored at 15 °C whereas total cyst germination and germ tube production ...