Peroxidase-mediated formation of corn fiber gum-bovine serum albumin conjugates: Molecular and structural characterization
Peroxidase-mediated formation of corn fiber gum-bovine serum albumin conjugates: Molecular and structural characterization
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过氧化物酶介导的玉米纤维胶-牛血清白蛋白缀合物的形成：分子和结构表征
DOI：
10.1016/j.carbpol.2017.02.069
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发表时间：
2017
影响因子：
11.2
通讯作者：
Yin Lijun
中科院分区：
文献类型：
--
作者：
Liu Yan;Yadav Madhav P.;Chau Hoa K.;Qiu Shuang;Zhang Hui;Yin Lijun
玉米纤维胶（CFG）经过氧化物酶处理与牛血清白蛋白（BSA）偶联以改善其乳化性能。本研究对CFG/BSA质量比为10:1、4:1、1:1和1:4时制备的CFG-BSA偶联物的分子特性进行了评价。过氧化物酶处理后，质量比为10:1和4:1的CFG-BSA混合物的分子量分别从200和193 kDa增加到218和223 kDa。而当CFG-BSA质量比为1:4时，其分子量减小。扫描电镜显示，与未处理过氧化物酶处理的CFG-BSA混合物相比，表面形貌更致密，但更粗糙。在较低的CFG与BSA质量比（1:4）下，CFG-BSA共轭物的颗粒呈细长状，而在较高的CFG与BSA质量比（10:1）下，它们在透射电子显微镜下呈近似球形。
Corn fiber gum (CFG) was previously conjugated with bovine serum albumin (BSA) via peroxidase treatment to improve its emulsifying properties. The current study was undertaken to evaluate the molecular characteristics of CFG-BSA conjugates prepared at different CFG/BSA weight ratios of 10:1, 4:1, 1:1, and 1:4. After the peroxidase treatment, CFG-BSA mixtures at weight ratios of 10:1 and 4:1 showed an increase in molecular weight from 200 and 193 kDa to 218 and 223 kDa respectively. But for CFG-BSA mixtures at weight ratio of 1:4, their molecular weight decreased. Peroxidase treated CFG-BSA mixtures had a more compact but rougher surface morphology as compared with the untreated as revealed by scanning electron microscopy. At lower CFG to BSA weight ratio (1:4), particles of CFG-BSA conjugates appeared to be elongated while at higher CFG to BSA weight ratio (10:1), they were approximately spherical in transmission electron micrographs.