Stimulation of pancreatic acinar cell growth by CCK, epidermal growth factor, and insulin in vitro.
Stimulation of pancreatic acinar cell growth by CCK, epidermal growth factor, and insulin in vitro.
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CCK、表皮生长因子和胰岛素体外刺激胰腺腺泡细胞生长。
DOI：
10.1152/ajpgi.1986.251.4.g487
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Logsdon,CD
中科院分区：
文献类型：
--
作者：
Logsdon,CD
观察调节分子对体外培养的小鼠胰腺腺泡细胞生长的影响。CCK类似物(CCK)和八肽(CCK-8)均可使DNA中[~3H]胸腺嘧啶核苷的掺入量增加三倍。胃泌素与CCK受体的相互作用很弱，但只能在更高的浓度下刺激DNA合成。相反，其他利用钙离子作为细胞内信使的促分泌剂，包括卡巴胆碱、蛙皮素、P物质和离子载体A23187，不能诱导营养反应。影响细胞内cAMP浓度的因素，如促胰液素、生长抑素、血管活性肠肽、DBcAMP和Forsklin，不能增加培养的胰腺细胞的DNA合成。胰岛素和表皮生长因子分别诱导DNA的[~3H]胸腺嘧啶核苷掺入增加2倍和3倍。胰岛素的作用是通过胰岛素样生长因子I受体介导的。类固醇激素对胰腺腺泡细胞DNA合成影响不大。CCK、胰岛素和EGF的刺激作用是相加的。在激素限定的培养液中，结合雨蛙素、EGF和胰岛素，可使DNA中的[~3H]胸腺嘧啶核苷的掺入增加10倍。这些数据表明，CCK、EGF和胰岛素直接增加了胰腺腺泡细胞的DNA合成。
Effects of regulatory molecules on growth of mouse pancreatic acinar cells in culture were examined. The cholecystokinin (CCK) analogue caerulein and cholecystokinin octapeptide (CCK-8) each led to threefold increases in incorporation of [3H]thymidine into DNA. Gastrin, which interacts weakly with the CCK receptor, stimulated DNA synthesis, but only at much higher concentrations. In contrast, other secretagogues that utilize Ca2+ as an intracellular messenger, including carbachol, bombesin, substance P, and the ionophore A23187, did not induce trophic responses. Factors that affect intracellular cAMP concentration, such as secretin, somatostatin, VIP, DBcAMP, and forskolin, did not increase DNA synthesis in cultured pancreatic cells. Insulin and epidermal growth factor induced two- and threefold increases in [3H] thymidine incorporation into DNA, respectively. The effects of insulin were mediated via insulin-like growth factor I receptors. Steroid hormones had little effect on pancreatic acinar cell DNA synthesis. The stimulatory effects of CCK, insulin, and EGF were additive. The combination of caerulein, EGF, and insulin in a hormonally defined medium led to a tenfold increase in the incorporation of [3H]thymidine into DNA. These data indicate that CCK, EGF, and insulin directly increase DNA synthesis in pancreatic acinar cells.