Crystallization and preliminary crystallographic analysis of a calcineurin B-like protein 1 (CBL1) mutant from Ammopiptanthus mongolicus.
Crystallization and preliminary crystallographic analysis of a calcineurin B-like protein 1 (CBL1) mutant from Ammopiptanthus mongolicus.
复制标题
沙冬青 (Ammopiptanthus mongolicus) 钙调神经磷酸酶 B 样蛋白 1 (CBL1) 突变体的结晶和初步晶体学分析。
DOI：
10.1107/s1744309110039667
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
R. Bi
中科院分区：
文献类型：
--
作者：
G. Shang;H. Cang;Zhijie Liu;W. Gao;R. Bi
钙调神经磷酸酶B样蛋白1（CBL1）是植物中的钙传感器。它通过下游蛋白CBL相互作用蛋白激酶（CIPK）传递钙信号。CBL 1和CIPK在植物对低K+、渗透压休克、高盐、寒冷和干旱等环境胁迫的应答中起着重要作用。对重组沙冬青CBL1（AmCBL1）进行了高效表达、纯化和结晶。然而，这种晶体并没有很好地发挥作用。用表面熵法制备突变体，并在298 K下用PEG 2000 MME作为沉淀剂用悬滴法结晶，衍射至2.90 nm分辨率。 晶体属P2（1）2（1）2空间群，晶胞参数a=99.87，B=114.42，c=63.80 °，α=β=γ=90.00°，每个不对称单元3个分子。
Calcineurin B-like protein 1 (CBL1) is a calcium sensor in plants. It transmits the calcium signal through the downstream protein CBL-interacting protein kinase (CIPK). CBL1 and CIPK play crucial roles in the response to environmental stresses such as low K+, osmotic shock, high salt, cold and drought. Recombinant CBL1 from Ammopiptanthus mongolicus (AmCBL1) was overexpressed, purified and crystallized. However, the crystal did not diffract well. A mutant prepared using the surface-entropy method and crystallized using the hanging-drop method at 298 K with PEG 2000 MME as a precipitant diffracted to 2.90 Å resolution. The crystal belonged to space group P2(1)2(1)2, with unit-cell parameters a=99.87, b=114.42, c=63.80 Å, α=β=γ=90.00° and three molecules per asymmetric unit.
影响因子：
16
作者：
Sanchez-Barrena, Maria Jose;Fujii, Hiroaki;Albert, Armando
通讯作者：
Albert, Armando
影响因子：
5.6
作者：
Sánchez-Barrena, MJ;Martínez-Ripoll, M;Albert, A
通讯作者：
Albert, A