Glucocorticoid-mediated induction of alpha 1-acid glycoprotein: evidence for hormone-regulated RNA processing.
Glucocorticoid-mediated induction of alpha 1-acid glycoprotein: evidence for hormone-regulated RNA processing.
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糖皮质激素介导的 α1-酸性糖蛋白诱导：激素调节 RNA 加工的证据。
DOI：
10.1073/pnas.81.14.4241
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发表时间：
1984
影响因子：
11.1
通讯作者：
Ringold,GM
中科院分区：
文献类型：
--
作者：
Vannice,JL;Taylor,JM;Ringold,GM
我们研究了 HTC 大鼠肝癌细胞中糖皮质激素介导的 mRNA 中 α1-酸性糖蛋白 (AGP) 的积累。与小鼠乳腺肿瘤病毒充分表征的初级反应相反，离体细胞核的体外转录测定表明，即使在没有激素的情况下，AGP 基因的转录率也很高。尽管 AGP 基因存在组成型转录，但在未处理细胞的细胞质或细胞核中均未发现可检测到的 AGP RNA。先前的实验表明，糖皮质激素诱导 AGP RNA 需要持续的蛋白质合成。结合本研究，我们的数据表明糖皮质激素通过诱导 RNA 加工因子来刺激 AGP RNA 的积累，从而产生稳定的转录本。
We have studied the glucocorticoid-mediated accumulation of alpha 1-acid glycoprotein (AGP) in mRNA in HTC rat hepatoma cells. In contrast to the well-characterized primary response of mouse mammary tumor virus, in vitro transcription assays in isolated nuclei show that the rate of transcription of the AGP gene is high even in the absence of hormone. Despite the constitutive transcription of the AGP gene, no detectable AGP RNA can be found in either the cytoplasm or the nuclei of untreated cells. Previous experiments have shown that the glucocorticoid induction of AGP RNA requires ongoing protein synthesis. In conjunction with the present study, our data suggest that glucocorticoids stimulate accumulation of AGP RNA by inducing an RNA processing factor that allows production of stable transcripts.