Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles.
Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles.
复制标题
光学计算相显微镜用于无标记细胞和纳米颗粒的定量动态成像。
DOI：
10.1364/boe.449034
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发表时间：
2022-01
影响因子：
3.4
通讯作者：
Xuan Liu;Zhaoxiong Wan;Yuanwei Zhang;Yuwei Liu
中科院分区：
文献类型：
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作者：
Xuan Liu;Zhaoxiong Wan;Yuanwei Zhang;Yuwei Liu
已经开发了许多基于纳米颗粒的药物递送系统，例如用于BioNTech/Pfizer和Moderna的Covid疫苗的那些。关于细胞和纳米颗粒之间的机械相互作用的知识对于提高这些药物递送系统的效率和安全性至关重要。为了定量跟踪细胞（透明）和纳米粒子（不透明）的运动与纳米位移灵敏度，我们研究了一种新的成像技术，光学计算相位显微镜（OCPM），通过光学计算处理三维空间光谱数据。我们证明，OCPM有能力成像的细胞和磁性纳米粒子的运动，由外部磁场，定量和在正面平面的机械激发。
Numerous drug delivery systems based on nanoparticles have been developed, such as those used in BioNTech/Pfizer's and Moderna's Covid vaccines. Knowledge on mechanical interactions between cells and nanoparticles is critical to advance the efficiency and safety of these drug delivery systems. To quantitatively track the motion of cell (transparent) and nanoparticles (nontransparent) with nanometer displacement sensitivity, we investigate a novel imaging technology, optically computed phase microscopy (OCPM) that processes 3D spatial-spectral data through optical computation. We demonstrate that OCPM has the capability to image the motion of cells and magnetic nanoparticles that are mechanically excited by an external magnetic field, quantitatively and in the en face plane.