Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins
Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins
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DOI：
10.1016/j.jsb.2012.02.012
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发表时间：
2012-05-01
影响因子：
3
通讯作者：
Ishikawa, Takashi
中科院分区：
文献类型：
--
作者：
Pigino, Gaia;Maheshwari, Aditi;Ishikawa, Takashi
虽然真核生物的鞭毛和纤毛都具有其内部轴丝的基本9+2微管组织，并能够产生弯曲运动，但弯曲运动的波形、幅度和速度是相当不同的。为了探索鞭毛和纤毛这种功能多样性的结构基础，我们用电子冷冻断层扫描技术比较了三种弯曲运动差异很大的不同生物的轴丝结构。我们重建了纤毛四膜虫轴丝的三维结构，并将其与海胆精子鞭毛的轴丝以及我们先前分析的衣藻鞭毛的轴丝进行了比较。这种比较结构分析定义了这些生物体中分子结构的多样性，并形成了未来与它们不同的弯曲运动相关联的基础。(C)2012 Elsevier Inc.保留所有权利。
Although eukaryotic flagella and cilia all share the basic 9 + 2 microtubule-organization of their internal axonemes, and are capable of generating bending-motion, the waveforms, amplitudes, and velocities of the bending-motions are quite diverse. To explore the structural basis of this functional diversity of flagella and cilia, we here compare the axonemal structure of three different organisms with widely divergent bending-motions by electron cryo-tomography. We reconstruct the 3D structure of the axoneme of Tetrahymena cilia, and compare it with the axoneme of the flagellum of sea urchin sperm, as well as with the axoneme of Chlamydomonas flagella, which we analyzed previously. This comparative structural analysis defines the diversity of molecular architectures in these organisms, and forms the basis for future correlation with their different bending-motions. (C) 2012 Elsevier Inc. All rights reserved.