Cloning and characterization of DNA complementary to the measles virus mRNA encoding hemagglutinin and matrix protein
Cloning and characterization of DNA complementary to the measles virus mRNA encoding hemagglutinin and matrix protein
复制标题
与编码血凝素和基质蛋白的麻疹病毒 mRNA 互补的 DNA 的克隆和表征
作者：
S. Rozenblatt;C. Gesang;V. Lavie;F. Neumann
自从克隆和鉴定了编码麻疹病毒核衣壳蛋白（M. Gorecki和S. Rozenblatt，Proc，Natl. Acad，Sci，U.S.A.77：3686- 3690，1980），已经鉴定了另外两种含有不同序列类别的麻疹特异性克隆。琼脂糖凝胶电泳和电子显微镜显示克隆的质粒含有480和530个碱基对的插入片段。通过北方技术测定，与这些插入物互补的mRNA种类的大小分别为1，700和1，550个核苷酸。克隆的DNA片段进一步鉴定为编码麻疹病毒糖蛋白和基质蛋白的mRNA的反转录产物。通过与单个克隆DNA杂交选择的mRNA的主要无细胞翻译产物与70 K体外产物和基质蛋白共迁移。其中一种无细胞翻译产物（70 K）也与麻疹病毒糖蛋白的单克隆抗体特异性免疫沉淀。
Since cloning and characterization of DNA complementary to measles virus mRNA encoding for the nucleocapsid protein (M. Gorecki and S. Rozenblatt, Proc, Natl. Acad, Sci, U.S.A. 77:3686--3690, 1980), two additional measles-specific clones containing different classes of sequences have been characterized. The cloned plasmids contain inserts of 480 and 530 base pairs as shown by agarose gel electrophoresis and electron microscopy. The sizes of the mRNA species complementary to these inserts are 1,700 and 1,550 nucleotides, respectively as determined by the Northern technique. The cloned DNA fragments were further identified as reverse transcripts of the mRNA coding for the glycoprotein and matrix protein of measles virus. The major cell-free translation products of mRNA selected by hybridization to the individual cloned DNAs comigrated with the 70K in vitro products and matrix proteins. One of the cell-free translation products (70K) was also immunoprecipitated specifically with monoclonal antibodies against measles virus glycoprotein.