Adding Emulsified Isoflurane to Cardioplegia Solution Produces Cardiac Protection in a Dog Cardiopulmonary Bypass Model.
Adding Emulsified Isoflurane to Cardioplegia Solution Produces Cardiac Protection in a Dog Cardiopulmonary Bypass Model.
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在心麻痹溶液中添加乳化异氟烷可在狗心肺旁路模型中产生心脏保护作用
DOI：
10.1038/srep23572
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发表时间：
2016-04-28
影响因子：
4.6
通讯作者：
Qiu Y
中科院分区：
文献类型：
--
作者：
Huang H;Zhou C;Liu J;Song H;Qiu Y
本研究在与临床非常相似的犬体外循环模型中研究了含乳化异氟醚（EI）的心肌麻痹液是否能改善心脏保护作用。成年犬随机分配接受以下心脏停搏液之一：St.托马斯与EI（ST+EI组）、St.托马斯与30% Intraperoid（ST+EL组）和St.托马斯单独（ST组）。将主动脉交叉夹闭2小时，然后再灌注2小时，在此期间测量心输出量并记录维持正常血流动力学的正性肌力药的剂量。检测血清心肌肌钙蛋白I（cTnI）和肌酸激酶同工酶（CK-MB）水平。在再灌注结束时检测心肌线粒体DNA的缺失。与ST相比，ST+EI降低了多巴胺支持的需求，而接受ST+EI的动物具有显著更大的心输出量。ST+EI降低CPB后cTnI和CK-MB的释放。ST+EI组仅1只动物出现线粒体DNA丢失，ST+EL组和ST组均出现线粒体DNA丢失。这种保护作用可能通过保护线粒体超微结构和DNA完整性来介导。
This study investigated whether caridoplegia solution with Emulsified Isoflurane (EI) could improve cardiaoprotection in a dog CPB model of great similarity to clinical settings. Adult dogs were randomly assigned to receive one of the following cardioplegia solutions: St. Thomas with EI (group ST+EI), St. Thomas with 30% Intralipid (group ST+EL) and St. Thomas alone (group ST). The aorta was cross-clamped for two hours followed by reperfusion for another two hours, during which cardiac output was measured and dosages of positive inotropic agent to maintain normal hemodynamics were recorded. Serum level of cardiac troponin I (cTnI) and CK-MB were measured. Deletion of cardiac mitochondrial DNA was examined at the end of reperfusion. Compared with ST, ST+EI decreased the requirement of dopamine support while animals receiving ST+EI had a significantly larger cardiac output. ST+EI reduced post-CPB release of cTnI and CK-MB. Mitochondrial DNA loss was observed in only one of the tested animals from group ST+EI while it was seen in all the tested animals from group ST+EL and ST. Addition of emulsified isoflurane into cardioplegia solution protects against myocardial ischemia reperfusion injury. This protective effect might be mediated by preserving mitochondrial ultrastructure and DNA integrity.