Expression of immediate early genes and vasopressin heteronuclear RNA in the paraventricular and supraoptic nuclei of rats after acute osmotic stimulus
Expression of immediate early genes and vasopressin heteronuclear RNA in the paraventricular and supraoptic nuclei of rats after acute osmotic stimulus
复制标题
DOI：
10.1111/j.1365-2826.2005.01297.x
复制
发表时间：
2005-04-01
影响因子：
3.2
通讯作者：
Ueta, Y
中科院分区：
文献类型：
--
作者：
Kawasaki, M;Yamaguchi, K;Ueta, Y
监测即刻早期基因(IEGs)的表达对于跟踪神经内分泌系统中应激诱导的细胞反应是有用的。用原位杂交组织化学方法检测了急性渗透刺激后大鼠下丘脑束旁核(PVN)和视上核(SON)中四种IEG(c-fos、JunB、NGFI-A和NGFI-B)和精氨酸加压素(AVP)基因的转录活性。腹膜后(Ip)给予高渗盐水(2%体重，900 mOsm/kg)后，PVN和SON中IEG mRNAs的表达水平在10min时显著升高，30min时达到高峰，并持续升高至60min。AVP异核(HN)RNA的表达也在30min达到高峰，并持续升高至180min。Ip后30分钟。给予高渗盐水(600mOsm/kg)后，PVN和SON中所有IEG mRNAs的表达水平均较ip后显著升高。注射等渗盐水(290mOsm/kg)。回归分析显示，IEG mRNAs和AVP hnRNA的表达水平与血钠浓度呈正相关，且所有IEG mRNAs表达水平的升高幅度相似。所有IEG mRNAs的表达水平可作为监测急性渗透刺激后PVN和SON中AVP基因转录变化的有用指标。
Monitoring the expression of immediate early genes (IEGs) is useful for following stress-induced cellular responses in the neuroendocrine system. We have examined the transcriptional activities of four IEGs (c-fos, junB, NGFI-A and NGFI-B) and of the arginine vasopressin (AVP) gene in the hypothalamic paraventicular (PVN) and supraoptic nuclei (SON) of rats after acute osmotic stimuli, using in situ hybridization histochemistry. After intraperitoneal (i.p.) administration of hypertonic saline (2% body weight, 900 mOsm/kg), the expression levels of all IEG mRNAs were increased significantly both in the PVN and SON at as early as 10 min, peaked at 30 min and remained elevated until 60 min. The expression of AVP heteronuclear (hn)RNA also peaked at 30 min, and remained elevated until 180 min. Thirty min after i.p. administration of hypertonic saline (600 mOsm/kg), the expression levels of all IEG mRNAs in the PVN and SON were significantly increased in comparison with those after i.p. administration of isotonic saline (290 mOsm/kg). Regression analysis revealed that expression levels of the IEG mRNAs and AVP hnRNA were positively correlated with the plasma concentration of sodium, and the rates of increase of the expression levels of all IEG mRNAs were similar. The expression levels of all IEG mRNAs examined are useful markers for following the changes of the AVP gene transcription in the PVN and SON after acute osmotic stimuli in rats.