Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
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DOI：
10.1073/pnas.81.8.2431
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发表时间：
1984-04
影响因子：
11.1
通讯作者：
G. Simchen;F. Winston;C. Styles;G. Fink
中科院分区：
文献类型：
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作者：
G. Simchen;F. Winston;C. Styles;G. Fink
在酿酒酵母的LYS 2位点分离到五个Ty插入突变。遗传和物理分析表明，4个Ty插入位于LYS 2的5'非编码区，1个位于结构基因内。其中三个Ty元件已被克隆和表征。Ty突变在限制性模式、对LYS 2的表型效应、回复频率和回复事件的性质方面彼此不同。已知抑制HIS 4处的Ty插入及其单独δ衍生物的Spt 2和spt 3突变也可以抑制LYS 2处的至少一个Ty插入（Ty 61），并且还可以抑制LYS 2处的另一个Ty插入（Ty 128）的单独δ衍生物的Lys-表型。这些结果表明，spt突变可以抑制在HIS 4和LYS 2的Ty和δ突变，表明它们对Ty和δ元件的影响是普遍的。
Five Ty insertion mutations were isolated at the LYS2 locus of Saccharomyces cerevisiae. Genetic and physical analyses show that four Ty insertions are in the 5' noncoding region of LYS2 and one is within the structural gene. Three of these Ty elements have been cloned and characterized. The Ty mutations differ from each other in restriction pattern, phenotypic effects on LYS2, reversion frequency, and the nature of reversion events. Spt2 and spt3 mutations, known to suppress Ty insertions and their solo delta derivatives at HIS4, can also suppress at least one of the Ty insertions (Ty61) at LYS2 and can also suppress the Lys- phenotype of a solo delta derivative of another Ty insertion (Ty128) at LYS2. These results demonstrate that spt mutations can suppress Ty and delta mutations at both HIS4 and LYS2, suggesting that they are general for their effects on Ty and delta elements.