Broad-spectrum resistance to Bacillus thuringiensis toxins by western corn rootworm (Diabrotica virgifera virgifera)
Broad-spectrum resistance to Bacillus thuringiensis toxins by western corn rootworm (Diabrotica virgifera virgifera)
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DOI：
10.1038/srep27860
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发表时间：
2016-06-14
影响因子：
4.6
通讯作者：
Gassmann, Aaron J.
中科院分区：
文献类型：
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作者：
Jakka, Siva R. K.;Shrestha, Ram B.;Gassmann, Aaron J.
抗性和交叉抗性的进化威胁到产生苏云金芽孢杆菌(Bt)杀虫毒素的转基因作物的可持续性。玉米根虫Diabrotica virgifera virgifera LeConte是一种严重危害玉米的害虫，自2003年开始使用Bt玉米进行防治。我们对产生Bt毒素Cry3Bb1、mCry3A、eCry3.1Ab和Cry34/35Ab1的玉米杂交种进行了实验室生物检测，这些毒素代表了所有商品化的Bt毒素，用于防治西部玉米根虫。我们测试了来自观察到对Cry3Bb1玉米严重伤害的田间种群，以及从未接触过Bt玉米的种群。与以往的研究一致，生物测定表明田间群体对Cry3Bb1玉米和mCry3A玉米都有抗性，并且这两种类型的Bt玉米之间存在交叉抗性。生物测定表明，玉米对eCry3.1Ab有抗性，而Cry3Bb1、mCry3A和eCry3.1Ab之间存在交叉抗性。但对Cry34/35Ab1玉米未检测到抗性或交叉抗性。这种广谱抗性说明了当Bt毒素之间存在结构相似时，害虫对多种Bt毒素迅速产生抗性的潜力，并引起了人们对Bt作物用于管理某些害虫的长期持久性的担忧。
The evolution of resistance and cross-resistance threaten the sustainability of genetically engineered crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a serious pest of maize and has been managed with Bt maize since 2003. We conducted laboratory bioassays with maize hybrids producing Bt toxins Cry3Bb1, mCry3A, eCry3.1Ab, and Cry34/35Ab1, which represent all commercialized Bt toxins for management of western corn rootworm. We tested populations from fields where severe injury to Cry3Bb1 maize was observed, and populations that had never been exposed to Bt maize. Consistent with past studies, bioassays indicated that field populations were resistant to Cry3Bb1 maize and mCry3A maize, and that cross-resistance was present between these two types of Bt maize. Additionally, bioassays revealed resistance to eCry3.1Ab maize and cross-resistance among Cry3Bb1, mCry3A and eCry3.1Ab. However, no resistance or cross-resistance was detected for Cry34/35Ab1 maize. This broad-spectrum resistance illustrates the potential for insect pests to develop resistance rapidly to multiple Bt toxins when structural similarities are present among toxins, and raises concerns about the long-term durability of Bt crops for management of some insect pests.