Characterization of gene expression profiles in HBV-related liver fibrosis patients and identification of ITGBL1 as a key regulator of fibrogenesis.
Characterization of gene expression profiles in HBV-related liver fibrosis patients and identification of ITGBL1 as a key regulator of fibrogenesis.
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HBV 相关肝纤维化患者基因表达谱的表征以及 ITBBL1 作为纤维发生关键调节因子的鉴定
DOI：
10.1038/srep43446
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发表时间：
2017-03-06
影响因子：
4.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Wang M;Gong Q;Zhang J;Chen L;Zhang Z;Lu L;Yu D;Han Y;Zhang D;Chen P;Zhang X;Yuan Z;Huang J;Zhang X
虽然B型肝炎病毒（HBV）感染是导致肝纤维化（LF）的主要原因，但肝纤维化进展的机制仍不清楚。在这里，我们调查了HBV相关LF患者的基因表达谱。全基因组表达阵列用于检测慢性HBV感染患者肝活检样本中的基因表达。通过综合数据分析，我们确定了参与肝纤维化发生和加重的几个途径和关键基因。体重基因共表达分析显示，整合素亚基β样1（ITGBL 1）是纤维化的关键调节因子。功能实验表明ITGBL 1通过与转化生长因子β1相互作用而成为LF的上游调控因子。总之，我们研究了HBV相关LF患者的基因表达谱，并确定了一个关键调节因子ITGBL 1。我们的研究结果为未来的基因功能研究提供了基础，并促进了新的抗纤维化疗法的发展。
Although hepatitis B virus (HBV) infection is the leading cause of liver fibrosis (LF), the mechanisms underlying liver fibrotic progression remain unclear. Here, we investigated the gene expression profiles of HBV-related LF patients. Whole genome expression arrays were used to detect gene expression in liver biopsy samples from chronically HBV infected patients. Through integrative data analysis, we identified several pathways and key genes involved in the initiation and exacerbation of liver fibrosis. Weight gene co-expression analysis revealed that integrin subunit β-like 1 (ITGBL1) was a key regulator of fibrogenesis. Functional experiments demonstrated that ITGBL1 was an upstream regulator of LF via interactions with transforming growth factor β1. In summary, we investigated the gene expression profiles of HBV-related LF patients and identified a key regulator ITGBL1. Our findings provide a foundation for future studies of gene functions and promote the development of novel antifibrotic therapies.