3-Decanol in the haemolymph of the hermit crab Clibanarius vittatus signals shell availability to conspecifics
3-Decanol in the haemolymph of the hermit crab Clibanarius vittatus signals shell availability to conspecifics
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DOI：
10.1016/j.jembe.2009.10.001
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发表时间：
2009-12-31
影响因子：
2
通讯作者：
Harder, Tilmann
中科院分区：
文献类型：
--
作者：
Schmidt, Gesine;Rittschof, Dan;Harder, Tilmann
寄居蟹与不适合壳化学吸引垂死的腹足类和同种在壳可能成为可用。对于陆地寄居蟹来说，壳线索是血淋巴中发现的挥发性化合物。基于这一知识，我们测试的假设，壳调查行为水生寄居蟹，陆生寄居蟹的祖先，也是由挥发性的线索。从水-血淋巴溶液中清除来自Clibanarius vittatus和Pagurus pollicaris和Brachyuran十足类甲壳动物血淋巴的挥发性化合物，将其捕获在海水中，并测试对幼C. vittatus。只有C.血淋巴刺激贝壳调查。采用顶空固相微萃取（SPME）技术分离血淋巴中的挥发性成分，并采用气相色谱-质谱联用（GC-MS）技术进行分析。鉴定出两个主要化合物，3-癸醇，这是C. vittatus血淋巴和2-乙基-1-己醇，其存在于所有4种甲壳动物的血淋巴中。在贝壳调查生物测定中，3-癸醇从C vittatus血淋巴刺激贝壳调查行为，而2-乙基-1-己醇没有。在用合成的1-、2-、4-和5-癸醇进行的生物测定中，1-癸醇、5-癸醇和3-十一烷醇引起壳调查行为。对2-和4-癸醇无反应。的C vittatus挥发性壳线索的反应支持的假设，挥发性线索检测进化之前的陆地生态位的甲壳类动物的占领。(C)2009 Elsevier B. V.保留所有权利。
Hermit crabs with poor fitting shells are chemically attracted to dying gastropods and conspecifics where a shell may become available. For land hermit crabs, the shell cue is a volatile compound found in the haemolymph. Based on this knowledge, we tested the hypothesis that shell investigation behavior in aquatic hermit crabs, the ancestral predecessors of terrestrial hermit crabs, is also triggered by volatile cues. Volatile compounds from haemolymph of Clibanarius vittatus and Pagurus pollicaris and brachyuran decapod crustaceans were purged from a water-haemolymph solution, trapped in seawater and tested for induction of shell investigation behavior with juvenile C. vittatus. Only volatiles from C. vittatus haemolymph stimulated shell investigation. Volatile compounds were isolated from haemolymph by headspace solid-phase microextraction (SPME) and analyzed by coupled gas chromatography-mass spectrometry (GC-MS). Two prominent compounds were identified, 3-decanol, which was unique to C. vittatus haemolymph, and 2-ethyl-1-hexanol which was present in the haemolymph of all 4 crustacean species. In shell investigation bioassays, 3-decanol from C vittatus haemolymph stimulated shell investigation behavior, while 2-ethyl-1-hexanol did not. In bioassays with synthetic 1-, 2-, 4-, and 5-decanol, shell investigation behavior was evoked by 1-decanol, 5-decanol and 3-undecanol. There was no response to 2- and 4-decanol. The response of C vittatus to volatile shell cues supports the hypothesis that volatile cue detection evolved prior to the occupation of terrestrial niches by crustaceans. (C) 2009 Elsevier B.V. All rights reserved.