Radiation induced DNA damage and damage repair in human tumor and fibroblast cell lines assessed by histone H2AX phosphorylation
Radiation induced DNA damage and damage repair in human tumor and fibroblast cell lines assessed by histone H2AX phosphorylation
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DOI：
10.1016/j.ijrobp.2005.09.037
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发表时间：
2006-02-01
影响因子：
7
通讯作者：
Djuzenova, CS
中科院分区：
文献类型：
--
作者：
Mahrhofer, H;Bürger, S;Djuzenova, CS
Purpose: To analyze the radiation-induced levels of gamma H2AX and its decay kinetics in 10 human cell lines covering a wide range of cellular radiosensitivity (SF2, 0.06-0.63).Methods and Materials: Five tumor cell lines included Colo-800 melanoma, two glioblastoma (MO59J and MO59K), fibrosarcoma HT 1080, and breast carcinoma MCF7. Five primary skin fibroblasts lines included two normal strains, an ataxia telangiectasia strain, and two fibroblast strains from breast cancer patients with an adverse early skin reaction to radiotherapy. Cellular radiosensitivity was assessed by colony-forming test. Deoxyribonucleic acid damage and repair were analyzed according to nuclear gamma H2AX foci intensity, with digital image analysis.Results: The cell lines tested showed a wide degree of variation in the background intensity of immunostained nuclear histone gamma H2AX, which was higher for the tumor cell lines compared with the fibroblast strains. It was not possible to predict clonogenic cell survival (SF2) for the 10 cell lines studied from the radiation-induced gamma H2AX intensity. In addition, the slopes of the dose-response (0-4 Gy) curves, the rates of gamma H2AX disappearance, and its residual expression (
