Inhibition of tubuloglomerular feedback during adenosine1 receptor blockade.
Inhibition of tubuloglomerular feedback during adenosine1 receptor blockade.
复制标题
腺苷 1 受体阻断期间肾小管肾小球反馈的抑制。
DOI：
10.1152/ajprenal.1990.258.3.f553
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Briggs,JP
中科院分区：
文献类型：
--
作者：
Schnermann,J;Weihprecht,H;Briggs,JP
在麻醉大鼠中进行实验，研究选择性腺苷 1 (A1) 受体拮抗剂 8-环戊基-1,3-二丙基黄嘌呤 (CPX) 对肾小球反馈 (TGF) 反应的影响，该反应评估为停流压力 (PSF) 的最大变化。与对照相比，在管腔内应用 10(-4) 和 10(-5)M 的 CPX 期间（分别为-4.9 +/- 0.44 与 + 0.9 +/- 0.42 mmHg 和-6.8 +/- 0.69 与 -1.4 +/- 0.7 mmHg），在管周施用 10(-4)M 的 CPX 期间（-6.2 +/- 0.44 对比 -2.8 +/- 0.42 mmHg），以及将 10(-4) M 的 CPX 输注到邻近肾单位管腔期间（-5.6 +/- 0.6 对比 -1.98 +/- 0.51 mmHg）。通过研究 CPX 对 A1 受体激动剂 N6-环己基腺苷 (CHA) 产生的 PSF 减少的影响来测试 CPX 的选择性。当注入管周血液时，10(-5)M 的 CHA 会降低 PSF (-11.8 +/- 3.7 mmHg)，而管腔应用 CPX (-1.5 +/- 0.6 mmHg) 会减弱这种效应。当注入邻近肾单位时，CHA 也会降低 PSF，并且通过将 10(-4)M CPX 注入同一邻近肾单位、不同邻近肾单位或管周毛细血管，可以减弱这种效应。这些结果与传入小动脉血管细胞上 A1 受体的激活参与介导 TGF 反应的概念一致。
Experiments were performed in anesthetized rats to study the effect of the selective adenosine1 (A1) receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (CPX) on tubuloglomerular feedback (TGF) responses assessed as the maximum change of stop-flow pressure (PSF). Compared with control, PSF responses were reduced during luminal application of CPX at 10(-4) and 10(-5)M (-4.9 +/- 0.44 vs. + 0.9 +/- 0.42 mmHg and -6.8 +/- 0.69 vs. -1.4 +/- 0.7 mmHg, respectively), during peritubular administration of CPX at 10(-4)M (-6.2 +/- 0.44 vs. -2.8 +/- 0.42 mmHg), and during infusion of CPX at 10(-4) M into the lumen of a neighboring nephron (-5.6 +/- 0.6 vs. -1.98 +/- 0.51 mmHg). Selectivity of CPX was tested by studying its effect on the PSF reduction produced by the A1-receptor agonist N6-cyclohexyladenosine (CHA). CHA at 10(-5)M reduced PSF when infused into the peritubular blood (-11.8 +/- 3.7 mmHg), and this effect was blunted by luminal application of CPX (-1.5 +/- 0.6 mmHg). CHA also reduced PSF when infused into a neighboring nephron, and this effect was blunted by infusing CPX at 10(-4)M into the same neighboring nephron, a different neighboring nephron, or a peritubular capillary. These results are consistent with the concept that activation of A1-receptors on vascular cells of the afferent arterioles participates in the mediation of TGF responses.